Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-05-10
2005-05-10
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C435S254200, C435S254210, C536S023100, C536S024100, C530S350000
Reexamination Certificate
active
06890730
ABSTRACT:
The invention provides a novel protein that, when expressed in a host cell, increases cellular biomass and cellular protein production and secretion. The invention also provides a polynucleotide encoding the novel protein, and methods for using the polynucleotide and encoded protein to increase cellular biomass and cellular protein production and secretion. Also provided is a genetically altered mutant cell strain with enhanced production and secretion of cellular and heterologous proteins.
REFERENCES:
patent: 4775622 (1988-10-01), Hitzeman et al.
patent: 5436136 (1995-07-01), Hinnen et al.
patent: 5672487 (1997-09-01), Schweden et al.
patent: 5834262 (1998-11-01), Anton et al.
Livingstone-Zatchej et al. Nuc. Acid Res. 25: 3795-3800, 1997.*
Everett et al..Nature Genetics 17: 411-422, 1997.*
Scott et al. Nature Genetics 21: 440-443, 1999.*
Johnston et al. Cloning and characterization of theSchizosaccharomyces pombeDNA ligase gene CDC17. 1986. Gene 41:321-325.*
Pagan-Ramos et al. Replacement of theSaccharomyces cerevisiaeRPR1 gene with heterologous RNase P RNA genes. Jan. 25, 1994. Nuc. Acids. Res. 22:200-207.*
Madison et al. Identification and analysis of homologues ofSaccharomyces cerevisiaeSpt3 suggest conserved functional domains. Mar. 30, 1998. Yeast 14:409-417.*
Mukai et al. Conservation of histone binding and transcriptional repressor functions in aSchizosaccharomyces pombeTup1p homolog. Dec. 1999. Mol. Cell. Biol. 19:8461-8468.*
Romanos et al., Yeast 8: 423-488, Foreign gene expression in yeast: a review, 1992.*
Zhou et al. Identification of a novel high affinity copper transport complex in the fusion yeastSchizosaccharomyces pombe. Jun. 8, 2001. Journal of Biological Chemistry. 276:20529-20535.*
EMBL entry EMBL:SCL9476.*
Mannhaupt, et al. Characterization of the prephenate dehydrogenase-encoding gene, TYR1, fromSaccharomyces cerevisiae. Dec. 28, 1989. Gene 85(2): 303-311.*
Powell et al. Molecular Biology of the Cell 10(supplement):298a, abstract No. 1727, Nov. 1999.*
Brake, A.J., et al., “α-factor-directed synthesis and secretion of mature foreign proteins inSaccharomyces cerevisiae”, PNAS, 81,pp. 4642-4646, (Aug. 1984).
Eakle, K.A., et al., “Characterization of a Component of the Yeast Secretion Machinery: Identification of the SEC18 Gene Product”,Molecular and Cellular Biology, 8(10), pp. 4098-4109, (Oct. 1988).
Fernandez-Lafuente, R., et al., “Chemoenzymatic One-pot Synthesis of Cefazolin Cephalosporin C in Fully Aqueous Medium, Involving Three Consecutive Biotransformations Catalyzed by D-Aminoacid Oxidase, Glutaryl Acylase and Penicillin G Acylase”,Tetrahedron Letters, 38(26), pp. 4693-4696, (1997).
Gavagan, J.E., et al., “Chemoenzymic Synthesis of N- (Phosphonomethyl) glycine”,J. Org. Chem., 62(16), pp. 5419-5427, (Aug. 1997).
Griff, I.C., et al., “The Yeast SEC17 Gene Product Is Functionally Equivalent to Mammalian α-SNAP Protein”,The Journal of Biological Chemistry, 267(17), pp. 12106-12115, (Jun. 15, 1992).
Lehninger, A.L., “Chapter 4: The Amino Acid Building Blocks of Proteins”,Biochemistry, Second Edition,Worth Publishers, Inc., pp. 73-75, (1975).
Morin-Ganet, M.N., et al., “Role of Endoplasmic Reticulum-Drived Vesicles in the Formation of Golgi Elements in sec23 and sec18Saccharomyces CerevisiaeMutants”,The Anatomical Record, 251(2), pp. 256-264, (1998).
Patel, S., et al., “The AAA Team: Related ATPases With Diverse Functions”,Trends in Cell Biology, 8,pp. 65-71, (Feb. 1998).
Powell, K.S., et al., “Vff2p, a nuclear sec17/sec18 suppressor in vesicular traffic and fusion”,Mol. Biol. Cell, 10 Suppl., Membrane Fusion Abstracts,Abstract No. 1727, p. 298a, (Nov. 1999).
Pryer, N.K., et al., “Vesicle-Mediated Protein Sorting”,Annu. Rev. Biochem., 61,pp. 471-516, (1992).
Sakaguchi, M., “Eukaryotic Protein Secretion”,Current Opinion in Biotechnology, 8,pp. 595-601, (1997).
Schweizer, F.E., et al., “Regulation of Neurotransmitter Release Kinetics by NSF”,Science, 279,pp. 1203-1206, (Feb. 1998).
Segev, N., et al., “The Yeast GTP-Binding YPT1 Protein and a Mammalian Counterpart Are Associated with the Secretion Machinery”,Cell, 52,pp. 915-924, (Mar. 1988).
Shaulsky, G., et al., “Development signal transduction pathways uncovered by genetic suppressors”,PNAS, 93,pp. 15260-15265, (Dec. 1996).
Steel, G.J., et al., “Biochemical Analysis of theSaccharomyces cerevisiaeSEC18 Gene Product: Implications for the Molecular Mechanism of Membrane Fusion”,Biochemistry, 38,pp. 7764-7772, (1999).
Stryer, L., “Introduction to Protein Structure and Function”,Biochemistry, Second Edition,Chapter 2, W.H. Freeman and Company, San Francisco, CA, pp. 14-15, (1981).
Tanaka, K., et al., “Characterization of a Fission Yeast SUMO-1 Homologue, Pmt3p, Required for Multiple Nuclear Events, Including the Control of Telomere Length and Chromosome Segregration”,Molecular and Cellular Biology, 19(12), pp. 8660-8672, (Dec. 1999).
Witt, W., et al., “Secretion of Phospholipase B FromSaccharomyces Cerevisiae”, Biochimica et Biophysica Acta, 795,pp. 117-124, (1984).
Woodman, P.G., et al., “Functional Conservation of Cytosolic Proteins Required for Endosomal Vesicle Fusion”,Yeast, 12,pp. 1251-1262, (1996).
Zsebo, K.M., et al., “Protein Secretion fromSaccharomyces cerevisiaeDirected by the Prepro-α-factor Leader Region”,The Journal of Biological Chemistry, 261(13), pp. 5858-5865, (May 5, 1986).
“AFG1 Protein—Yeast (Saccharomyces Cerevisiae)”, NCBI Protein S30825.
“agCP10084 [Anopheles Gambiae Str. PEST]”,EAA07345.
“Alpha-soluble NSF Attachment Protein [Arabidopsis Thaliana]”, NCBI Protein NP 191178.
“Alpha-soluble NSF Attachment Protein-like [Arabidopsis thaliana]”, NCBI Protein—NP 191204.
“ATPase”,NCBI Protein AAA34414.
“BLASTP 2.2.3 [Apr. 24, 2002]”.
“BLASTP 2.2.4 [Aug. 26, 2002]”.
“BRCA2 and CDKN1A-Interacting Protein Isoform BCCIPalpha; BRCA2 and CDKN1A-interacting Protein; cdk Inhibitor p21 binding protein; BCCIPalpha; BCCIPbeta; TOK-1alpha; TOK-1beta [Homo Sapiens]”,NCBI Protein—NP 057651.
“Hypothetical ORF; Bcp1p [Saccharomyces Cerevisiae]”, NCBI Protein—NP 010648.
“Hypothetical Protein [Schizosaccharomyces Pombe]”,NCBI Protein—NP 587696.
“Hypothetical Protein [Schizosaccharomyces Pombe]”, NCBI Protein—CAA21060.
“Nsf; N-ethylmaleimide Sensitive Factor [Rattus Norvegicus]”, NCBI Protein—NP 068516.
“SEC18 Protein—Yeast (Saccharomyces Cerevisiae)”,NCBI Protein S45477.
“Unknown”,NCBI Protein AAB60280.
“Vesicular-fusion protein NSF (N-ethylmameimide-sensitive fusion protein) (NEM-sensitive fusion protein)”,NCBI Protein P46459.
“Vesicular-fusion Protein NSF [Caenorhabditis Elegans]”, NCBI Protein NP 492152.
“Vesicular-fusion Protein Nsf1 (N-ethylmaleimide-sensitive Fusion Protein 1) (NEM-sensitive Fusion Protein 1) (DNsf-1) (Comatose Protein)”,NCBI Protein—P46461.
“Vesicular-fusion Protein Nsf2 (N-ethylmaleimide-sensitive Fusion Protein 2) NEM-sensitive Fusion Protein 2) (DNsf-2)”,NCBI Protein—P54351.
“Vesicular-fusion Protein SEC18”,NCBI Protein P34732.
“Vesicular-fusion Protein Sec18.”,NCBI Protein P18759.
Latterich Martin
Powell Kendall
Ketter James
Lambertson David
McDermott Will & Emery LLP
The Salk Institute
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