Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-07
1999-10-19
Jones, W. Gary
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435325, 536 237, 935 9, 935 55, 935 70, 935 72, C12P 2100, C12N 516, C12N 701, C12N 1531
Patent
active
059687690
ABSTRACT:
The invention relates to the isolation and cloning of the structural gene, hipP, for the NTHi pili serotype 5 and the LKP operon. The invention relates to DNA molecules capable of hybridizing to the DNA sequences of the Haemophilus influenzae genome related to the pili. The invention further relates to a DNA molecule which encodes a pili protein, particularly a tip adhesion protein. The DNA molecules of the invention can be used in a method for assaying a sample, such as a blood sample, for the presence of Haemophilus influenzae in the sample. Accordingly, the invention further relates to the use of the DNA molecules as a diagnostic. The invention also relates to a recombinant Haemophilus influenzae pili protein, such as a tip adhesion protein. The protein can be employed in a method for immunizing an animal, such as a human, as a therapeutic or diagnostic.
REFERENCES:
patent: 4801690 (1989-01-01), Brinton, Jr. et al.
patent: 5336490 (1994-08-01), Brinton, Jr. et al.
patent: 5399481 (1995-03-01), McMillan et al.
Watson, Wendy, J. et al., "Identification of a Gene Essential for Piliation in Haemophilus influenzae Type b with Homology to the Pilus Assembly Platform Genes of Gram-Negative Bacteria," Infection and Immunity 62(2):468-475 (1994).
van Ham, S. M. et al., "Phase Variation of H. influenzae Fimbriae: Transcriptional Control of Two Divergent Genes through a Variable Combined Promoter Region," Cell 73:1187-1196 (1993).
van Alphen, L. et al., "Blocking of Fimbria-Mediated Adherence of Haemophilus influenzae by Sialyl Gangliosides," Infection and Immunity 59(12):4473-4477 (1991).
Strom, M.S. et al., "A Single Bifunctional Enzyme, PilD, Catalyzes Cleavage and N-methylation of Proteins Belonging to the Type IV Pilin Family," Proc. Natl. Acad. Sci. USA 90:2404-2408 (1993).
St. Geme, J.W., III et al., "High-Molecular-Weight Proteins of Nontypable Haemophilus influenzae Mediate Attachment to Human Epithelial Cells," Proc. Natl. Acad. Sci. USA 90:2875-2879 (1993).
Sinha, N.D. et al., "Polymer Support Oligonucleotide Synthesis XVIII.sup.1.2: Use of .beta.-cyanoethyl-N, N-dialkylamino-/N-morpholino Phosphoramidite of Deoxynucleosides for the Synthesis of DNA Fragments Simplifying Deprotection and Isolation of the Final Product," Nucleic Acids Research 12(11):4539-4557 (1984).
Saiki, R.K. et al., "Primer-Directed Enzymatic Amplification of DNA With a Thermostable DNA Polymerase," Science 239:487-490 (1988).
Palmer, K.L. and Munson, R.S., Jr. "Construction of Chimaeric Genes for Mapping a Surface-Exposed Epitope on the Pilus of Non-typable Haemophilus influenzae Strain M37," Molecular Microbiology 6(18):2583-2588 (1992).
Musher, D.M. et al., "Pneumonia and Acute Febrile Tracheobronchitis Due to Haemophilus influenzae," Annals of Internal Medicine 99:444-450 (1983).
Miller, J.H. "Generalized Transduction; Use of P1 in Strain Construction," Experiments in Molecular Genetics, pp. 201-205 Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1972).
McCaman, M.T. et al., "Genetics and Regulation of Peptidase N in Escherichia coli K-12," Journal of Bacteriology 152(2):848-854 (1982).
Laemmli, U.K., "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4," Nature 227:680-685 (1970).
Karasic, R.B. et al., "Evaluation of Pilus Vaccines for Prevention of Experimental Ptitis Media Caused by Nontypable Haemophilus influenzae," Pediatr. Infect. Dis. J 8(1):S062-S065 (1989).
Kar, S. et al., "Cloning and Expression in Escherichia coli of LKP Pilus Genes from a Nontypeable Haemophilus influenzae Strain," Infection and Immunity 58(4):903-908 (1990).
Gilsdorf, J.R., "Cloning, Expression, and Sequence Analysis of the Haemophilus influenzae Type b Strain M43p.sup.+ Pilin Gene," Infection and Immunity 58(4):1065-1072 (1990).
Forney, L.J. et al., "Comparison and Analysis of the Nucleotide Sequences of Pilin Genes from Haemophilus influenzae Type b Strains Eagan and M43," Infection and Immunity 59(6):1991-1996 (1991).
Coleman, T. et al., "Molecular Cloning, Expression, and Sequence of the Pilin Gene from Nontypeable Haemophilus influenzae M37," Infection and Immunity 59(5):1716-1722 (1991).
Chang, A.C.Y. and Cohen, S.N. "Construction and Characterization of Amplifiable Multicopy DNA Cloning Vehicles Derived from the P15A Cryptic Miniplasmid," Journal of Bacteriology 134(3):1141-1156 (1978).
Brinton, C.C., Jr. et al., "Design and Development of Pilus Vaccines for Haemophilus influenzae Diseases," Pediatr. Infect. Dis. J. 8(1):S54-S61 (1989).
Bluestone, C.D. and Klein, J.O., "Otitis Media With Effusion, Atelectasis, and Eustachian Tube Dysfunction," Pediatric Otolaryngology pp. 356-512 (1983).
Haslam, D. et al., "The Amino-Terminal Domain of the P-Pilus Adhesion Determines Receptor Specificity," Journal of Cellular Biochemistry Supplement 18A:47, Abstract B112 (1994).
Langermann S. and Wright A., "Molecular Analysis of the Haemophilus Influenzae Type b Pilin Gene,", Molecular Microbiology, 4(3) :221-230 (1990).
van Ham, S.M. et al., "The Fimbrial Gene Cluster of Haemophilus Influenzae Type b," Molecular Microbiology, 13(4) :673-684 (1994).
Lindberg, F. et al., "Gene Products Specifying Adhesion of Uropathogenic Escherichia Coli Are Minor Components of Pili," Proc. Natl. Acad. Sci. USA, 83:1891-1895 (1986).
Watson, W.J., et al., "Identification of a Gene Essential for Piliation in Haemophilus Influenzae Type b With Homology to the Pilus Assembly Platform Genes of Gram-Negative Bacteria," Database EMBL/GenBank/DDBJ on STRAND, ID=HIIFC, AC=U02932 (1994).
Smith, A.L., "hif B of H. Influenzae is a Member of the Chaperone Family," Database EMBL/GenBank/DDBJ on STRAND, ID=HIHIFB, AC=X66606 (1992).
VanHam, S.M., et al., "Cloning and Expression in Escherichia Coli of Haemophilus Influenzae Fimbral Genes Establishes Adherence to Oropharyn-Geal Epithelial Cells," EMBO J., 8:3535-3540 (1989).
Green, B.A., et al., "Sequence of LKP Serotype 5 hifA Gene," Database EMBL/GenBank/DDBJ on STRAND, ID=HII9795, AC=U19795 (1995).
Langerman and Wright, Molecular Microbiology 4: 221-230, Cited in SN 08277231, 1990.
Sambrook et al. In Molecular Cloning: A Laboratory Manual, Cited in SN 08277231, 1989.
Brinton, Jr. Charles C.
Green Bruce A.
American Cyanamid Co. and Bactex, Inc.
Jones W. Gary
Shoemaker Debra
LandOfFree
Sequence and analysis of LKP pilin structural genes and the LKP does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Sequence and analysis of LKP pilin structural genes and the LKP , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sequence and analysis of LKP pilin structural genes and the LKP will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2054585