Sequence alterations using homologous recombination

Details Sequence alterations using homologous recombination Sequence alterations using homologous recombination

1997-08-13

1999-09-07

Degen, Nancy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 911, 530350, 536 231, C07H 2104, C07K 1400, C12N 1500, C12P 1934

Patent

active

059486538

ABSTRACT:
The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell. The invention also relates to compositions that contain exogenous targeting polynucleotides, complementary pairs of exogenous targeting polynucleotides, chemical substituents of such polynucleotides, and recombinase proteins used in the methods of the invention.

REFERENCES:
patent: 4873191 (1989-10-01), Wagner et al.
patent: 4888274 (1989-12-01), Radding et al.
patent: 4950599 (1990-08-01), Bertling
patent: 5223414 (1993-06-01), Zarling et al.
patent: 5264618 (1993-11-01), Felgner et al.
patent: 5272071 (1993-12-01), Chappel
patent: 5273881 (1993-12-01), Sena et al.
patent: 5416260 (1995-05-01), Koller et al.
patent: 5451513 (1995-09-01), Maliga et al.
patent: 5459072 (1995-10-01), McKay et al.
patent: 5460941 (1995-10-01), Camerini-Otero et al.
patent: 5464764 (1995-11-01), Capecchi et al.
patent: 5468629 (1995-11-01), Calhoun
patent: 5487992 (1996-01-01), Capecchi et al.
patent: 5501967 (1996-03-01), Offringa et al.
patent: 5506098 (1996-04-01), Zarling et al.
patent: 5510473 (1996-04-01), Camerini-Otero et al.
patent: 5527674 (1996-06-01), Guerra et al.
patent: 5565350 (1996-10-01), Kmiec
patent: 5571688 (1996-11-01), Mekalanos et al.
patent: 5578461 (1996-11-01), Sherwin et al.
patent: 5580734 (1996-12-01), Treco et al.
patent: 5589369 (1996-12-01), Seidman et al.
patent: 5612205 (1997-03-01), Kay et al.
patent: 5614396 (1997-03-01), Bradley et al.
Gates, C.M., et al., Affinity Selective Isolation of Ligands from Peptide Libraries Through Display on a lac Repressor "Headpiece Dimer", J. Mol. Biol., 255:373-386 (1996).
Rashid, N., et al., "Characterization of a RecA/RAD51 homologue from the hyperthermophilic archaeon Pyrococcus sp KOD1", Nucleic Acids Research, vol. 25, No. 4:719-726 (1997).
Jayasena, V.K., et al., "Complement-stabilized D-loop--RecA-catalyzed Stable Pairing of Linear DNA Molecules at Internal Sites", J. Mol. Biol., 230:1015-1024 (1993).
Crameri, A., et al., "Construction and evolution of antibody-phage libraries by DNA shuffling", Nature Medicine, vol.2, No. 1:100-102 (Jan. 1996).
Cole-Strauss, A., et al., "Correction of the Mutation Responsible for Sickle Cell Anemia by an RNA-DNA Oligonucleotide", Science, vol.273:1386-1389 (Sep. 1996).
Matsumura, I., et al., "DNA Shuffling brightens prospects for GFP", Nature Bio Tech., vol. 14:366 (Mar. 1996).
Stemmer, W.P.C., "DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution", Proc. Natl. Acad. Sci. USA, vol. 91:10747-10751 (Oct. 1994).
Meyer Jr., R.B., et al., "Efficient, Specific Cross-Linking and Cleavage of DNA by Stable, Synthetic Complementary Oligodeoxynucleotides", J. of the Amer. Chem. Soc., 111(22):8517-8519 (1989).
Kido, M., et al., "Escherichia coli RecA Protein Modified with a Nuclear Location Signal Binds to Chromosomes in Living Mammalian Cells", Exper. Cell Res., 198:107-114 (1992).
Woo, J., et al., "G/C-modified oligodeoxynucleotides with selective complementarity: synthesis and hybridization properties", Nucleic Acids Res., 24(13):2470-2475 (1996).
Kowalczykowski, S.C., et al., "Homologous Recombination Proteins and their Potential Applications in Gene Targeting Technology," Gene Targeting, CRC Press: Boca Raton, ed. Manuel A. Vega, Chap. 7:167-210 (1995).
Kunzelmann, K., et al., "Gene targeting of CFTR DNA in CF epithelial cells", Gene Therapy, 3:859-867 (1996).
Voloshin, O.N., et al., "Homologous DNA pairing promoted by a 20 amino acid peptide derived from RecA", Science, 272:868-872 (1996).
Revet, B.M.J., et al., "Homologous DNA Targeting with RecA Protein-coated Short DNA Probes and Electron Microscope Mapping on Linear Duplex Molecules", rep. fr. J. Mol. Biol., 232:779-791 (1993).
Camerini-Otero, R.D., et al., "Homologous Recombination Proteins in Prokaryotes and Eukaryotes", Annu. Rev. Genetics, 29:509-52 (1995).
Crameri, A., et al., "Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling", Nature BioTech., 14:315-319 (Mar. 1996).
Kutyavin, I.V., et al., "Oligonucleotides Containing 2-Aminoadenine and 2-Thiothymine Act as Selectively Binding Complementary Agents", Biochemistry, 35:11170-11176 (1996).
Herzing, L.B.K., et al., "Novel lacZ-based recombination vectors for mammalian cells", Gene, 137:163-169 (1993).
Stemmer, W.P.C., "Rapid evolution of a protein in vitro by DNA shuffling", Nature, 370:389-391 (Aug. 1994).
Reiss, B., et al., "RecA protein stimulates homologous recombination in plants", Proc. Natl. Acad. Sci. USA, 93:3094-3098 (Apr. 1996).
Podyminogin, M.A., et al., "RecA-Catalyzed, Sequence-Specific Alkylation of DNA by Cross-Linking Oligonucleotides. Effects of Length and Nonhomologous Base Substitutions", Biochemistry, 35:7267-7274 (1996).
Bertolotti, R., "Recombinase-mediated Gene Therapy", Bionews, Newsletter of BioTechnology, Health and Environmental Sciences, N14 (Nov. 1996).
Afonina, I., et al., "Sequence-specific arrest of primer extension on single-stranded DNA by an oligonucleotide-minor groove binder conjugate", Proc. Natl. Acad. Sci. USA, 93:3199-3204 (Apr. 1996).
Podyminogin, M.A., et al., "Sequence-Specific Covalent Modification of DNA by Cross-Linking Oligonucleotides. Catalysis by RecA and Implication for the Mechanism of Synaptic Joint Formation", Biochemistry, 34:13098-13108 (1995).
Pati, W., et al., "Sequence-Specific DNA Targeting", Encyclo. of Cancer, vol. III:1601-1625 (1997).
Fu, D., et al., "Sequencing double-stranded DNA by strand displacement", Nucleic Acids Res., 25(3):677-679 (1997).
Hunger-Bertling, K., et al., "Short DNA fragments induce site specific recombination in mammalian cells", Mol. and Cellular Biochem., 92:107-116 (1990).
Stemmer, W.P.C., et al., "Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides", Gene, 164:49-53 (1995).
Ludwig, D.L., et al., "Spontaneous and Induced Homologous Recombination Between lacZ Chromosomal Direct Repeats in CV-1 Cells", Somane Cell and Molecular Genetics, 20(1):11-25 (1994).
Yoon, K., et al., "Targeted gene correction of episomal DNA in mammalian cells mediated by a chimeric RNA-DNA oligonucleotide", Proc. Natl. Acad. Sci. USA, 93:2071-2076 (Mar. 1996).
Jasin, M., et al., "Targeted transgenesis", Proc. Natl. Acad. Sci. USA, 93:8804-8808 (Aug. 1996).
Rawls, R., "Hybrid DNA-RNA efficiently repairs gene", C&EN, p.11 (Sep. 1996).
Sena, E.P., et al., "Targeting in linear DNA duplexes with two complementary probe strands for hybrid stability", Nature Genetics, 3:365-372 (Apr. 1993).
Lukhtanov, E.A. et al., "Rapid and Efficient Hybridization-Triggered Crosslinking within a DNA Duplex by an Oligodeoxyribonucleotide Bearing a Conjugated Cyclopropapyrroloindole," Nucleic Acids Research, 24(4):683-687 (1996).
Tabone, J.C. et al., "Factors Influencing the Extent and Regiospecificity of Cross-Link Formation between Single-Stranded DNA and Reactive Complementary Oligodeoxynucleotides," Biochemistry, 33(1):375-383 (1994).
Bertling, "Transfection of a DNA/Protein Complex into Nuclei of Mammalian Cells Using Polyoma Capsids and Electroporation," Bioscience Reports, 7:107-111 (1987).
Ausubel et al., "Short Protocols in Molecular Biology," 2n ed. (John Wiley & Sons: New York), pp. 9-14 and 9-15 (1992).
Wu et al., "Targeting Genes: Delivery and Persistent Expression of a Foreign Gene Driven by Mammalian Regulatory Elements in Vivo,"

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