Separation process

Details Separation process Separation process

1989-06-30

1990-03-20

Mays, Thomas D.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Lysis of micro-organism

435134, 435141, 435271, 435829, 435874, C12N 106, C12P 752, C12P 764

Patent

active

049101455

ABSTRACT:
An aqueous suspension of micro-organism cells containing a 3-hydroxybutyrate polymer are subjected to a proteolytic enzyme digestion and/or a surfactant digestion in order to solubilise cell material other than the 3-hydroxybutyrate polymer.
Prior to, or during the digestion, but before any proteolytic enzyme digestion step, the suspension is heated to at least 80.degree. C. to denature nucleic acids which otherwise hinder separation of the 3-hydroxybutyrate polymer containing residue from the suspension.

REFERENCES:
patent: 3275610 (1966-09-01), Coty
patent: 3928642 (1975-12-01), Hubert et al.
patent: 4138291 (1979-02-01), Lafferty
patent: 4358583 (1982-11-01), Walker et al.
patent: 4562245 (1985-12-01), Stageman
Krieg et al. eds. In Bergey's Manual of Systematic Bacteriology, 9th Ed., vol. pp. 34-35.
Merrick et al. Journal of Bacteriology (1964), vol. 88, No. 1, pp. 60-71, Schlegel Allugemeine Microbiologie, Georg Thiene Verlag Stuttgart (1976), p. 38, FIG. 25, Martin et al., Zeit. for Naturforschung, (1962), 17b, pp. 190-196.
Braun et European J. Biochem. (1969) 10: 426-438.
Biochemistry, vol. 7, No. 10, Oct. 1986, pp. 3676-3681; R. Griebel et al.; "Metabilism of Poly-Beta-Hydroxybutyrate., I. Purification, Composition, and Properties of Native Poly-Beta-Hydroxybutyrate Granules from Bacillus Megaterium".
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