Separation of proteins by hydrophobic adsorption

Details Separation of proteins by hydrophobic adsorption Separation of proteins by hydrophobic adsorption

1974-09-10

1976-12-28

Woodberry, Edward M.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

195 63, 195 66R, 195 68, 195127, 195DIG11, 210 24, 260112R, C07G 700

Patent

active

040000982

ABSTRACT:
Separation of proteins from one another by passing an aqueous solution of a mixture of proteins through two or more bodies of matrices each having hydrophobic ligands covalently attached to it, the order of hydrophobicity being such that the protein mixture comes into contact with a first body having the least hydrophobic ligands, then with a second body having more hydrophobic ligands, etc., whereby the most highly hydrophobic proteins are adsorbed on the first (least hydrophobic) matrix, etc. in order of increasing hydrophobicity of the matrices and decreasing hydrophobicity of the proteins in the mixture.

REFERENCES:
patent: 3562289 (1971-02-01), Battista et al.
patent: 3705084 (1972-12-01), Reynolds
El-el, et al., "Hydrocarbon - Coated Sepharoses;" Bio-Chem. and Biophys. Res. Comm. vol. 49, No. 2, 1972(pp. 383-390).
Yon; R. J., "Enzyme Purification by Hydrophobic Chromatography" Bio-Chem. J., vol. 137, Jan. 1974(pp. 127-130).
Hofstee; B. H. J., "Protein Bonding by Agarose Carrying Hydrophobic Groups in Conjunction with Charges;" Bio-Chem. and Biophys., Res. Comm., vol. 50 No. 3, Feb. 1973 (pp. 751-757).
Zaborsky; O., "Immobilized Enzymes" CRC Press; Cleveland, Ohio, May 1973, (pp.75-82).
C.A. 75:118613d, "Adsorbents for Chromatographic Protein Separation," Fryklund et al., 1971.
C.A. 78:133023s, "Hydrophobic Affinity Chromatography of Proteins," Hofstee, 1973.

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