Separation of proteases from fluids

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435176, 435183, 435188, 435815, C12N 950, C12N 1114

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active

043189903

ABSTRACT:
Proteases are separated from fluids by contacting a fluid with a porous inorganic material such as porous titania particles to sorb the protease and then eluting the protease from the inorganic material. Proteases which may be separated are the sulphydryl proteases including papain, stem bromelain, fruit bromelain, ficin, calotropain and mexicain. Elution of the protease may be carried out with plant juice from which protease has previously been removed. The porous inorganic material is preferably that prepared by mixing a finely divided sorptive inorganic material with a solid fugitive additive and a solvent to dissolve the fugitive, forming discrete particles from the resultant mixture and heating the particles to remove the solvent and fugitive to produce discrete particles of the inorganic material for contacting with the fluid.

REFERENCES:
patent: 3095358 (1963-06-01), Meister
patent: 3293143 (1966-12-01), Heinicke
patent: 3850751 (1974-11-01), Messing
patent: 3943072 (1976-03-01), Thomson et al.

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