Separation of polynucleotides using supports having a plurality

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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436 94, 436 72, 436807, 436809, 536 254, 422 681, 422101, C12Q 168, G01N 3348

Patent

active

054340497

ABSTRACT:
A method for simultaneously detecting a plurality of target polynucleotides in a sample on a single reaction chip and a method for separating a plurality of target polynucleotides are provided. On the reaction chip are arranged a plurality of independent cells for capturing different target polynucleotides. Different probes are immobilized onto the individual cells, and labeled probes detect the cells where the target polynucleotides are captured, thereby analyzing the species of the captured target polynucleotides. Also, the individual cells function as electrodes to elute the captured target polynucleotides therethrough, thereby separating a plurality of the target polynucleotides.

REFERENCES:
patent: 4859302 (1989-08-01), Alfenito
patent: 4945045 (1990-07-01), Forrest et al.
patent: 5089387 (1992-02-01), Tsay et al.

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