Separation of phosphorothioate oligonucleotides by capillary gel

Details Separation of phosphorothioate oligonucleotides by capillary gel Separation of phosphorothioate oligonucleotides by capillary gel

1992-12-16

1995-05-30

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 261, 536 2672, C07H 106, C07H 2104, C07H 1910, C07H 1920

Patent

active

054202655

ABSTRACT:
A method for separating a mixture of 5'-phosphorothioate mononucleotides, a mixture of 5'-phosphorothioate oligonucleotides, or a mixture of both 5'-phosphorothioate mononucleotides and 5'-phosphorothioate oligonucleotides comprising electro- phoresis through a gel of at least 12% polymerized linear acrylamide containing at least 50% formamide or other denaturing organic solvent. This method can resolve phosphorothioate oligonucleotides differing by only a single base. Samples of less than 1 nanogram in microliter volumes can be conveniently handled.

REFERENCES:
patent: 2713041 (1955-07-01), Friedlander et al.
patent: 4865706 (1989-09-01), Karger et al.
patent: 4865707 (1989-09-01), Karger et al.
patent: 5003059 (1991-03-01), Brennan
patent: 5098539 (1992-03-01), Shieh
patent: 5112460 (1992-05-01), Karger et al.
patent: 5183885 (1993-02-01), Bergot
Widhalm et al. (1991) J. Chromatog. 549:446-451.
Hjerten (1967) Chromatog. Rev. 9:122-219.
Burgers et al. (1979) Biochem. 18:592-596.
Edge et al. (1981) Nature 292:756-762.
Stec et al. (1985) J. Chromatog. 326:263-280.
Murakami et al. (1985) Biochem 24:4041-4046.
Agrawal et al. (1987) Tetrahedron Lett. 28:3539-3542.
Agrawal et al. (188) Proc. Natl. Acad. Sci. (USA) 85:7079-7083.
Sarin et al. (1988) Proc. Natl. Acad. Sci. (USA) 85:7448-7451.
Cohen et al. (1988) Proc. Natl. Acad. Sci. (USA) 85:9660-9663.
Agrawal et al. (1989) Nucleosides and Nucleotides 8:819-823.
Agrawal et al. (1988) Proc. Natl. Acad. Sci. (USA) 86:7790-7794.
Wu et al. (1989) J. Chromatog. 480:141-155.
Agrawal et al. (1990) J. Chromatog. 509:396-399.
Agrawal et al. (1990) Nucleic Acids. Res. 18:5419-5423.
Ansorge et al. (1990) Nucleic Acids. Res. 18:3419-3420.
Bigelow et al. (1990) J. Chromatog. 553:131-140.
Swerdlow et al. (1990) J. Chromatog. 516:61-67.
Cohen et al. (1990) J. Chromatog. 516:49-60.
Brumley et al. (1991) Nucleic Acids. Res. 19:4121-4126.
Chen et al. (1991) J. Chromatog. 559:237-246.
Swerdlow et al. (1991) Anal. Chem. 63:2835-2841.
Zhang et al. (1991) Clin. Chem. 39:1492-1496.
Bergot (1992) J. Chromatog. 599:35-42.
Meletev et al. (1992) Analyt. Biochem. 200:342-346.
Rocheleau et al. (1992) Electrophoresis 13:484-486.
Efcavitch in Gel Electrophoresis of Nucleic Acids, A Practical Approach (2d. Ed.) (Rickwood and Hames, eds.) IRL Press at Oxford University Press, New York, pp. 125-126.
Froehler (1986) Tetrahedron Lett. 27:5575-5578.
Garegg et al. (1986) Tetrahedron Lett. 27:4051-4054.
Heiger et al. (1990) J. Chromatogr. 516:33-48.
Guttman et al. (1991) Anal. Chem. 63:2038-2042.
Agrawal et al. (1992) TIBTECH 10:152-158.

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