Liquid purification or separation – Processes – Separating
Patent
1979-03-01
1981-01-20
Cintins, Ivars C.
Liquid purification or separation
Processes
Separating
210259, 210317, 210927, B01D 2506
Patent
active
042461073
ABSTRACT:
Lymphocytes are advantageously separated from blood or other lymphocyte-containing suspensions by a method wherein the lymphocyte-containing suspension is flowed through a second filter having a mass of fibers exhibiting an average fiber diameter of greater than 10 microns but not greater than 60 microns, thereby to entrap a substantial part of granulocytes and monocytes in the mass of fibers and to obtain a granulocyte-poor and monocyte-poor lymphocyte-containing suspension; and then, the obtained suspension is flowed through a first filter having a mass of fibers exhibiting a bulk density of 0.04 to 0.40 g/cm.sup.3 and an average fiber diameter of 5 to 20 microns, said average fiber diameter being less than that of the second filter, thereby to entrap a substantial part of lymphocytes in the mass of fibers; and finally, the lymphocytes entrapped in the mass of fibers of the first filter is collected.
Alternatively, another method is employed wherein the lymphocyte-containing suspension is flowed through the first filter thereby to entrap a substantial part of leukocyte components therein; and the leukocyte components entrapped in the first filter is collected to obtain a leukocyte component-rich suspension; and finally, the leukocyte component-rich suspension is flowed through the second filter thereby to entrap a substantial part of granulocytes and monocytes in the second filter and to obtain a lymphocyte-rich suspension.
REFERENCES:
patent: 2302552 (1942-11-01), Johnson
patent: 3448041 (1969-06-01), Swank
patent: 3954621 (1976-05-01), Etani et al.
patent: 4073723 (1978-02-01), Swank et al.
patent: 4116845 (1978-09-01), Swank
Kuroda Toru
Takenaka Yoshinori
Tsuda Nobuaki
Asahi Kasei Kogyo Kabushiki Kaisha
Cintins Ivars C.
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