Liquid purification or separation – Processes – Separating
Patent
1986-10-28
1989-03-28
Fisher, Richard V.
Liquid purification or separation
Processes
Separating
210516, 422101, 435 2, 436177, 494 37, B01D 2126
Patent
active
048161683
ABSTRACT:
This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.
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Carrol Richard J.
Fluderer Albert A.
Smith Ward C.
Zine, Jr. Anthony R.
Becton Dickinson & Company
Fisher Richard V.
Jones W. Gary
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