Liquid purification or separation – Processes – Separating
Patent
1984-12-24
1987-02-03
Fisher, Richard V.
Liquid purification or separation
Processes
Separating
210789, 435 2, B01D 2126
Patent
active
046407855
ABSTRACT:
This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.
REFERENCES:
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patent: 3920549 (1975-11-01), Gigliello et al.
patent: 4101422 (1978-07-01), Lamont et al.
patent: 4190535 (1980-02-01), Luderer et al.
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patent: 4436631 (1984-03-01), Graham, Jr. et al.
Carroll Richard J.
Luderer Albert A.
Smith Ward C.
Zine, Jr. Anthony R.
Becton Dickinson and Company
Fisher Richard V.
Jones W. Gary
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