Separation of large DNA molecules in alternating asymmetric elec

Details Separation of large DNA molecules in alternating asymmetric elec Separation of large DNA molecules in alternating asymmetric elec

1988-02-03

1989-05-16

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

G01N 2726

Patent

active

048307262

ABSTRACT:
A method of separating DNA molecules by gel electrophoresis is disclosed which employs alternate application of high and low strength electric fields in opposite directions to a gel matrix containing DNA. The high strength field is maintained for a shorter interval than the low strength field, and the ratio of the product of the low strength field and its pulse interval to the product of the high strength field and its pulse interval is greater than 4:1 and sufficient to produce a net migration of the DNA molecules in the direction of the low strength field.

REFERENCES:
patent: 4473452 (1987-09-01), Cantor
Schwartz et al., "Separation of Yeast Chromosome-Sized DNAs by Pulsed Field Gradient Gel Electrophoresis", Cell, vol. 37, pp. 67-75 (1984).
Carle et al., "Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric Field," Science, vol. 232, pp. 65-68 (1986).
Chu et al., "Separation of Large DNA Molecules by Contour-Clamped Homogenous Electric Fields", Science, vol. 234, pp. 1582-1585 (1986).
D. Vollrath et al, Nucleic Acids Res., 15(19), 7865-7876, (1987).
G. F. Carle and M. V. Olson, Nucleic Acids Res., 12: 5647-5664, (1984).
K. Gardiner et al., Som. Cell Mol. Genet., 12: 185-195, (1986).

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