Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1994-03-15
1996-05-14
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
426580, 435815, 530395, 530415, 530417, C12N 908, C07K 300, A23J 100, A23C 2300
Patent
active
055166750
ABSTRACT:
Lactoperoxidase, secretory component and lactoferrin are separated in high purity from milk and related materials such as whey with a single cation exchange resin. After adsorption on the cation exchange resin, elution is carried out with an aqueous solution having an ionic strength and pH selected to elute each separately. Lactoperoxidase is eluted first, secretory component second and lactoferrin last. Each is obtained in a purity of about 80% or greater. The cation exchange resin can be a cross-linked polysaccharide, cellulose or an acrylamide resin having carboxyl, sulfonic acid or phosphoric acid functional groups which may be attached with a spacer. The separated lactoperoxidase, secretory component and lactoferrin are biologically active and can be used in pharmaceuticals, cosmetics, foods, drinks and feeds.
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Dosako Shun'ichi
Kawasaki Yoshihiro
Sato Kaoru
Uchida Toshiaki
Naff David M.
Snow Brand Milk Products Co. Ltd.
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