Liquid purification or separation – Processes – Chromatography
Patent
1987-11-06
1989-03-07
Therkorn, Ernest G.
Liquid purification or separation
Processes
Chromatography
210635, 210139, 210143, 2101982, 422 70, 436 66, 436161, 530385, 530413, 530416, 530417, B01D 1508
Patent
active
048103918
ABSTRACT:
An ion exchange method for the separation of hemoglobin A.sub.2 from other hemoglobin components normally present in human blood involves the use of three buffer solutions in succession, with specified ionic strengths and pHs. The first has a phosphate buffer at 1 to 20 mM and a pH of 6.5 to 6.9; the second has phosphate buffer at 15 to 55 mM and a pH of 6.4 to 6.8; and the third has phosphate buffer at 60 to 100 mM and a pH of about 6.4 to 6.8. The first is continued until substantially all hemoglobin A.sub.1a and A.sub.1b has eluted from the column, the second until substantially all A.sub.1c, F, and A.sub.0 is eluted, and the third for the elution of A.sub.2.
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Diamat Fully Automated Glycosylated Hemoglobin Analyzer System, Instruction Manual, Nov. 1985.
Bio-Rad Laboratories, Inc.
Therkorn Ernest G.
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