Separation of different isoforms of plasmid DNA using...

Details Separation of different isoforms of plasmid DNA using... Separation of different isoforms of plasmid DNA using...

2011-08-02

2011-08-02

Crane, Lawrence E (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

Reexamination Certificate

active

07989615

ABSTRACT:
The invention is a method for isolating a desired plasmid DNA isoform, typically a supercoiled isoform, which includes the steps of obtaining a quantity of bioreactor produced plasmid DNA in liquid form for purification, and passing the plasmid DNA through an ultrafiltration membrane at a selected filtrate flux.

REFERENCES:
patent: 6444447 (2002-09-01), DeAngelis
patent: 7105327 (2006-09-01), Kuppusamy et al.
patent: 7238522 (2007-07-01), Hebel et al.
patent: 7285651 (2007-10-01), Lander et al.
patent: 7604973 (2009-10-01), DeAngelis
patent: 7767399 (2010-08-01), Murphy et al.
Varley et al., “Production of Plasmid DNA for Human Gene Therapy using Modified Alkaline Cell Lysis and Expanded Bed Anion Exchange Chromatography,” Bioseparation, 8, 209-217 (1999).
Latulippe, David R., et al., “Flux-dependent transmission of supercoiled plasmid DNA through ultrafiltration membranes,” Journal of Membrane Science 294 (2007) 169-177.
Kong, Simyee et al., “Plasmid DNA processing for gene therapy and vaccination: Studies on the membrane sterilisation filtration step,” Journal of Membrane Science 280 (2006) 824-831.
Hirasaki, Tomoko et al., “Permeation mechanism of DNA molecules in solution through cuprammonium regenerated cellulose hollow fiber (BMMtm),” Journal of Membrane Science 106 (1995) 123-129.

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