Separation of cells and biological macromolecules by ferritin co

Details Separation of cells and biological macromolecules by ferritin co Separation of cells and biological macromolecules by ferritin co

1992-01-16

1993-09-28

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

209214, 436526, 436801, 436806, 436828, C12N 506, G01N 33553

Patent

active

052485897

ABSTRACT:
An Fc receptor protein conjugated with Ferritin binds to an exposed Fc region of an antibody to form a cell/Ferritin complex. Magnetic particles are added to the medium and bind to the complex. The magnetic particles when bound to the complex significantly enhance the magnetic field gradient of the complex such that it may be separated magnetically from the medium.

REFERENCES:
patent: 3951785 (1976-04-01), Kaiser et al.
patent: 4375407 (1983-03-01), Kronick
patent: 4552773 (1984-06-01), Molday
Templeton et al., "Ferritin-Conjugated Protein A," FEBS Letters, vol. 85, No. 1, (1978), pp. 95-98.
A. L. Lehninger, Biochemistry, 2nd Edition, Worth Publishers, Inc., N.Y., p. 59, (1970).

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