Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2006-03-02
2008-12-02
Saidha, Tekchand (Department: 1652)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S416000, C530S418000
Reexamination Certificate
active
07459542
ABSTRACT:
The present invention generally relates to methods for separating albumin from samples comprising albumin and other proteins based on the pI of albumin. More particularly, the methods relate to forming an aqueous combination comprising the sample and an anion or a cation exchange material at various pHs, agitating the combination, and separating a fraction of unbound proteins from a concentrated solids fraction comprising the ion exchange material and bound proteins.
REFERENCES:
patent: 4228154 (1980-10-01), Fisher et al.
patent: 5250662 (1993-10-01), Chang
patent: 5888966 (1999-03-01), Larsen et al.
patent: 6489447 (2002-12-01), Basey et al.
Ziad J Sahab and Qing-Xiang A Sang, Albumin elimination from human plasma, Abstract, Division of Analytical Chemistry, Aug. 28-Sep. 1, 2005, ANYL 150, Washington, DC.
Ben Herbert, Pier Giorgio Righetti, A Turning point in proteome analysis: Sample prefractionation via multicompartment electrolyzers with isoelectric membranes, Electrophoresis, 3639-3648, 21, Wiley-VCH Veriag GmbH, 69451 Weinheim, 2000.
Radhakrishna S. Tirumalai, King C. Chan, Darue A. Prieto, Haleem J. Issaq, Thomas P. Conrads, and Timothy D. Veenstrat, Characterization of the Low Molecular Weight Human Serum Proteome, pp. 1096-1103, Molecular & Cellular Proteomics 2.10, Aug. 13, 2003.
Stanford Moore and William H. Stein, Chromatography of Amino Acids on Sulfonated Polystyrene Resins, Journal of Biological Chemistry, Oct. 1951;192(2) pp. 663-681.
2-D Electrophoresis for Proteomics, A Methods and Product Manual, BIO-RAD Laboratories, Inc., Bulletin 2651 US/EG Rev C.
Ziad J Sahab, Yewseok Suh, and Qing-Xiang A Sang, Pre-fractionation of proteins from crude biological samples prior to 2-D gel electrophoresis, Abstract, Division of Analytical Chemistry, Aug. 28-Sep. 1, 2005, ANYL 151, Washington, DC.
Lian Shan and David J. Anderson, Gradient Chromatofocusing. Versatile pH Gradient Separation of Proteins in Ion-Exchange HPLC: Characterization Studies, Analytical Chemistry, American Chemical Society, vol. 74, No. 21, Nov. 1, 2002, pp. 5641-5649.
Ziad J. Sahab, Yewseok Suh, and Qing-Xiang Amy Sang, Isoelectric Point-Based Prefractionation of Proteins from Crude Biological Samples Prior to Two-Dimensional Gel Electrophoresis, Journal of Proteome Research, American Chemical Society, 2005, 4, pp. 2266-2272.
Sahab Ziad Joseph
Sang Qing-Xiang (Amy)
Bryan Cave LLP
Florida State University Research Foundation, Inc.
Saidha Tekchand
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