Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1999-10-26
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435458, C12Q 168
Patent
active
059726008
ABSTRACT:
The invention separates defined, active complexes by a characteristic from Defined, active complexes that share a particular physicochemical characteristic such as density, surface charge or particle size are separated from complexes formed by the association of a polynucleotide with a transfecting component that increases transfection activity, such as a lipid, cationic lipid, liposome, peptide, cationic peptide, dendrimer or polycation. In a preferred embodiment, polynucleotide-transfecting component complexes are ultracentrifuged to resolve one or more bands corresponding to complexes having a specific polynucleotide-transfecting component interaction. Polynucleotide complexes having a cationic liposome transfecting component resolve into two primary bands corresponding to complexes formed either under excess lipid conditions or under excess polynucleotide conditions. In an alternate embodiment, polynucleotide-transfecting component complexes are resolved using cross-flow electrophoresis to identify complexes having specific interactions and to separate them from excess initial components.
REFERENCES:
Deshayes et al. EMBO Journal. vol. 4, No. 11, pp. 2731-2737, 1985.
Faust et al. Virology. vol. 168, pp. 128-137, 1989.
Kawano et al. Experimental Cell Research. vol. 161, pp. 460-472, 1985.
Shimizu et al. J. Biochem. vol. 98, pp. 1473-1485, 1985.
Witkiewicz et al. Febs Letters. vol. 90, No. 2, pp. 313-317, 1978.
Gershon et al. Mode of Formation and Structural Features of DNA-Cationic Liposome Complexes Used for Transfection. Biochemistry. 32: 7143-7151, 1993.
Alberts et al. Fractionation of Cell Contents in Molecular Biology of the Cell, 2nd edn., Garland Publishing, Inc., New York, 1989, pp. 163-165.
Szoka, Jr. Francis C.
Wang Jinkang
Xu Yuhong
Degen Nancy
Koenig Nathan P.
The Regents of the University of California
Yucel Irem
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