Separation-free specific binding assays using anti-inhibitor ant

Details Separation-free specific binding assays using anti-inhibitor ant Separation-free specific binding assays using anti-inhibitor ant

1994-05-31

1996-10-15

Spiegel, Carol A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435962, 435969, 435970, 435975, 436518, 436523, 436529, 436530, 436531, 436548, 436816, 436822, G01N 3353

Patent

active

055653260

ABSTRACT:
Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, a reporter enzyme, an inhibitor antibody and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti-inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the target specific binding ligand to the signal generated without the use of separation or wash steps.

REFERENCES:
patent: 3935074 (1976-01-01), Rubenstein et al.
patent: 4161515 (1979-07-01), Ullman
patent: 4233401 (1980-11-01), Yoshida et al.
patent: 4430263 (1984-02-01), Merch et al.
patent: 4621048 (1986-11-01), Ashihara et al.
patent: 4686181 (1987-08-01), Dona
Henderson et al, Clin. Chem., 32 (9), pp. 1637-1641 (1986).
Ngo, int. J. Biochem., 15(5), pp. 583-590 (1983).
Derwent Abstract 92/176890/22, Syntex USA Inc, "Stabilizing conjugate of enzyme and specific binding pair--using antibody for enzyme in assays for analytes", EP 487301, see entire abstract.

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