Separation and recovery of B and T lymphocytes

Liquid purification or separation – Processes – Ion exchange or selective sorption

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210927, B01D 1500

Patent

active

042565880

ABSTRACT:
A process for the separation of B and T lymphocytes comprising passing a suspension of a mixture over a bed of synthetic polymer granules whereby the B leukocytes adhere to the granules and the T lymphocytes pass through the bed.

REFERENCES:
patent: 2682268 (1954-06-01), Ryan et al.
patent: 3448041 (1969-06-01), Swank
patent: 3929130 (1975-12-01), Hargest
patent: 4050451 (1977-09-01), Columbus
patent: 4064042 (1977-12-01), Kunin
Trizio et al., "Separation of T and B Lymphocytes by Nylon Wool Columns: Evaluation of Efficacy by Functional Assays in Vivo", The Jouranl of _Immunology, vol, 113, No. 4, Oct. 1974, pp. 1093-1097.
Rabinowitz, Y. "Separation of Lymphocytes, Polymorphonuclear Leukocytes and Monocytes on Glass Columns, Including Tissue Culture Observations", Blood, vol. 23, No. 6 (Jun.), 1964, pp. 811-828.
Eisen et al., "Isolation of Pure Human Peripheral Blood T-Lymphocytes", Immunological Communications, 1(6), 1922, pp. 571-577.

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