Separation and isolation of conjugated and unconjugated bilirubi

Details Separation and isolation of conjugated and unconjugated bilirubi Separation and isolation of conjugated and unconjugated bilirubi

1979-12-10

1982-01-19

Turk, Arnold

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Calorimeter

23230B, 23905, 252408, 260112B, 542434, C07D20700, G01N 3372

Patent

active

043116655

ABSTRACT:
A method is described for the separation and isolation of conjugated and unconjugated bilirubin from an aqueous liquid containing one or both of the bilirubin components. The aqueous liquid is contacted together with an interactive mordant for bilirubin to mordant bilirubin, the mordanted bilirubin is separated from the aqueous liquid, and the mordanted bilirubin is treated in an aqueous medium with a chaotropic agent at least slightly soluble in the medium to release the conjugated and/or unconjugated bilirubin from the mordant. A preferred embodiment also provides for selectively separating conjugated bilirubin from unconjugated bilirubin by selectively solubilizing either the conjugated or unconjugated bilirubin released from the mordant. Isolated conjugated bilirubin is thereby obtained having a purity in excess of 75%. A reference composition comprising the isolated conjugated bilirubin is also disclosed.

REFERENCES:
patent: 4069017 (1978-01-01), Wu et al.
Abstract of Wu et al., "Human Conjugated Bilirubin-Isolation, Biosynthesis, & Molecular Characterization by Direct Spectroscopic Analyses" presented at the American Association for Clinical Chemistry 31st Annual Meeting in New Orleans, Louisiana, Jul. 15-20, 1979, Abstract appears in Clinical Chemistry, vol. 25, No. 6, p. 1137, (Jun. 1979).
J. Lucassen Doctoral Thesis, University of Utrecht, Netherlands, (1961).
With, Bile Pigments, Academic Press, New York, pp. 369-375, (1968).
Carey et al., "Self-Association of Unconjugated Bilirubin-Ix in Aqueous Solution at pH 10.0 and Physical-Chemical Interactions with Bile Salt Monomers and Micelles", Biochem. J. (1979), 179, 675-687.
Ostrow et al., Biochem. J. (1970), 120, 311-327.

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