Separating agent, separator and method of separating cell or vir

Details Separating agent, separator and method of separating cell or vir Separating agent, separator and method of separating cell or vir

1990-02-28

1992-02-04

Jones, W. Gary

Liquid purification or separation

Processes

Ion exchange or selective sorption

210290, 210492, 2105021, 210691, 210807, 422101, 436177, 435 2, 502402, 502404, B01D 1500, B01D 3902, B01D 3914

Patent

active

050857819

ABSTRACT:
A separating agent which includes a polyvinyl acetal resin having open cell structure and an average pore size of from about 10 to about 1000 micrometers; and, porous calcium phosphate granules having open cell structure, and also having two distributions of pore size wherein one distribution has micropores ranging from about 20 to about 500 nm in average pore size and the other distribution has small pores ranging from about 1 to about 50 micrometers in average pore size. Alternatively, the separating agent includes a polyvinyl acetal resin having open cell structure and having an average pore size of from about 10 to about 1000 micrometers as a first element, and a layer of porous calcium phosphate granules having an average granule size of from about 10 to about 2000 micrometers as a second element applied onto the first element. The separating agents may be used in a separator, and may be employed in separating methods. The separating agents, separator, and separating methods can be advantageously used to isolate a target cell or virus from a biological fluid in the fields of medical science and biochemistry. The distribution of target cell or virus subsets is not altered by the separation.

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Tsuru, S. et al., "A rapid method for the isolation of functional human T lymphocytes using hydroxy apatite column fractionation," Journal of Immunological Methods, 106:169-174 (1988).

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