Sensizitation of cancer cells to therapy using siNA...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C514S04400A, C435S320100

Reexamination Certificate

active

07939653

ABSTRACT:
The invention relates to the identification of genes involved in resistance of cancer cells to therapy, to short nucleic acid molecules which inhibit the expression of these genes by RNA interference and to their use as adjuvant in cancer therapy, to sensitize cancer cells to conventional anticancer agents; the short nucleic acid molecules are double-stranded short interfering nucleic acid molecules including a sense and an antisense region, wherein the sense region includes a nucleotide sequence that is selected from the group consisting of: the sequences SEQ ID NO: 15, 11, 13, 14, 30, 31, 38, 46, 64 and 70 and the sequences having at least 70% identity, preferably at least 80% identity, more preferably at least 90% identity with the sequences, and the antisense region includes a nucleotide sequence that is complementary to the sense region.

REFERENCES:
patent: 2003/0143732 (2003-07-01), Fosnaugh et al.
patent: 2004/0265230 (2004-12-01), Martinez et al.
patent: 2006/0134189 (2006-06-01), MacLachlan et al.
patent: 2007/0031844 (2007-02-01), Khvorova et al.
patent: 03/070918 (2003-08-01), None
patent: 2005/123953 (2005-12-01), None
Chang, I.Y. et al., “Small Interfering RNA-Induced Suppression of ERCC1 Enhances Sensitivity of Human Cancer Cells to Cisplatin”, Biochemical and Biophysical Research Communications, Feb. 4, 2005, pp. 225-233, vol. 237, No. 1, Academic Press Inc., Orlando, FL XP004697918.
Selvakumaran, M. et al., “Enhanced Cisplatin Cytotoxicity by Disturbing the Nucleotide Excision Repair Pathway in Ovarian Cancer Cell Lines”, Cancer Research, American Association for Cancer Research, Mar. 15, 2003, pp. 1311-1316, vol. 63, No. 6 XP002321157.
EP Search Report dated Jan. 7, 2007 from corresponding EP 06291241.

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