Sensitive radioimmunoassay using antibody to L-hyoscyamine

Details Sensitive radioimmunoassay using antibody to L-hyoscyamine Sensitive radioimmunoassay using antibody to L-hyoscyamine

1983-09-23

1986-05-27

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

424 85, 424 88, 436543, 436545, 436547, 436804, 436815, 436822, 530387, G01N 3356, A61R 3900, C07G 700

Patent

active

045915733

ABSTRACT:
A sensitive and specific radioimmunoassay is developed for L-hyoscyamine ulting from atropine administration with which a concentration as low as 25 pg/ml using 0.1 ml of sample can be measured without the need for extraction. Specificity studies indicate that an antibody according to this invention has high specific recognition of L-hyoscyamine with only about 37% cross reaction with the D-hyoscyamine enantiomer of atropine. The antibody is produced from an immunogen having conjugated at least 42 and preferably 45 L-hyoscyamine-p-aminobenzoic acid haptenic molecules per molecule of bovine serum albumin. An antibody according to this invention can be used with a dilution titer as high as 1:2000.

REFERENCES:
Virtanen et al, Chemical Abstracts, 93 (1980), #230464d.
Lehtola et al, Chemical Abstracts, 97 (1982), #205286y.
Wurzburger et al, J. Pharmacol. Exp. Therapeut., vol. 203 (1977), 435-41.

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