Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-02-24
1998-06-02
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 536 243, 536 2431, 536 2433, C12Q 168, C12P 1934, C07H 2104
Patent
active
057597731
ABSTRACT:
Nucleic acid sandwich hybridization assays are provided that incorporate one or a combination of background reduction steps. Those steps include use of a separate capture probe and separation from immobilized capture probes by cleavage and isolation. A very sensitive assay for RNA targets includes both of those steps, plus RNA binary probes, an RNA-directed RNA ligase and amplification by an RNA-directed RNA polymerase. Kits of reagents for performing assays according to this invention are also provided.
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4775619 (1988-10-01), Ureda
Blok, H.J., Target-Dependent Amplifiable Nucleic Acid Hybridization Probes. 992. Doctoral Thesis. Eindhoven University of Technology, Eindhoven, The Netherlands.
Krinke et al, "The cleavage specificity of RNase III", Nucleic Acids Res. 18(16):4809-4815, 1990.
Haseloff et al, "Simple RNA enzymes with new and highly specific endoribonuclease activities", Nature 334:585-591, Aug. 1988.
Gibco/BRL catalog, "SuperScript(tm) Choice system for cDNA synthesis", pp. 368-369, Feb. 1992.
Chu et al, Nucleic Acids Rev, V. M, N.Y, 1986, pp. 5591-5603.
Barary, Proc. Natl. Acad. Sci., V. 88, Jan. 1991, 189-93.
Matthews et al, Anal. Biochem, V. 169, 1988, 1-2 J.
Landegren et al, Science, v. 241, Aug. 1988, 1077-1080.
Korarska et al, Cell, v. 57 May 5, 1989, 423-31.
Blok Herman J.
Kramer Fred R.
Landegren Ulf D.
Lizardi Paul M.
Tyagi Sanjay
Fredman Jeffrey
The Public Health Research Institute of the City of New York, In
Zitomer Stephanie W.
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