Sensitive method for detecting low levels of ATP

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase

Reexamination Certificate

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Reexamination Certificate

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07494781

ABSTRACT:
Methods, devices and systems are provided for detection of adenosine triphosphate (ATP) in samples using the luciferin-luciferase reaction. Aspects include a low pH composition for use in detecting the presence of ATP. The low pH composition can include low molarity buffers and detergents and can be used in combination with methods for reading, calculating and interpreting luminescence generated by the ATP-luciferin-luciferase reaction. Both the low molarity and low pH composition, and the methods for reading, calculating and interpreting luminescence, can be used with a single service hygiene monitoring format.

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