Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-04-04
2000-07-04
Minnifield, Nita
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 75, 435 772, 435 791, 435 792, 435 9124, 435 9151, 435971, 435973, 436518, 436536, 436547, 436824, 436828, C12Q 168, G01N 3353, G01N 33536, G01N 33543
Patent
active
06083689&
ABSTRACT:
A novel signal amplification system for immunoassays that minimizes non-specific signals is disclosed. Immunoassay methods, reagents and test kits are described for obtaining immunoassays of enhanced sensitivity. The reagents include antibody-variant DNA conjugates, wherein the variant DNA is a substrate for an RNA-dependent RNA polymerase, such as, QB replicase. Immunoassay methods to detect, or to detect and quantitate, analyte in test samples comprise transcribing the variant DNA of said antibody-DNA conjugates that are bound to analyte, to RNA, and replicating the RNA transcripts, wherein the presence or quantity of variant RNA replication products can be correlated with the presence or quantity of analyte in the test samples. Further, methods are provided to detect, or to detect and quantitate, simultaneously two or more analytes in a test sample.
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Carroll, III Eddie
Martinelli Richard A.
Bayer Corporation
Lauder Leona L.
Masood Khalid
Minnifield Nita
Morgenstern Arthur S.
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