Sensitive and rapid diagnosis of viroid diseases and viruses

Details Sensitive and rapid diagnosis of viroid diseases and viruses Sensitive and rapid diagnosis of viroid diseases and viruses

1981-12-03

1984-10-30

Padgett, Benjamin R.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

Utilizing isolate of tissue or organ as binding agent

436 63, 436 64, 436 94, 436804, 436813, 436815, 435 6, 4351723, 435 91, 435270, 935 78, 935 64, 935 6, 935 29, 935 73, G01N 3350, C12N 1500

Patent

active

044800401

ABSTRACT:
A rapid and sensitive method for diagnosing plant viroid diseases and viruses. Plant sap is bound to a solid support and the bound sample probed with a radioactively labelled DNA that is complementary to the viroid or to the nucleic acid of the virus being diagnosed. The radioactively labelled cDNA hybridizes with that viroid or virus RNA or DNA for which it is specific. DNA-RNA and DNA-DNA hybrids are detected by autoradiographic examination of the hybridized material.

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