Sensitive and highly specific quantitative fluorometric assay fo

Details Sensitive and highly specific quantitative fluorometric assay fo Sensitive and highly specific quantitative fluorometric assay fo

1995-05-02

1996-06-18

Warden, Jill

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-n

436 96, 436106, 436164, 436169, 436172, 422 56, 422 8208, G01N 3300

Patent

active

055277080

ABSTRACT:
A quantitative assay for creatinine has been developed in which creatinine is reacted with 3,5-dinitrobenzoate under alkaline reaction conditions to produce a fluorophore product which has excitation and emission maxima near 410 nm and 475 nm, respectively. Alternatively, 3,5-dinitrobenzoyl chloride or methyl-3,5-dinitrobenzoate may be employed as reagents. The chemical reactions proceed in the presence of Group IA bases and/or quaternary alkyl ammonium hydroxides under aqueous, nonaqueous and mixed solvent conditions. Detection limits for creatinine are well below one micromole per liter. Conversely, the reactivity of creatinine and/or base with 3,5-dinitrobenzoate may be employed to quantify 3,5-dinitrobenzoate by fluorescence intensity measurements. The chemical assay is readily adaptable to reagent pad and dry-layered coating technologies employing fluorescence detection/quantitation.

REFERENCES:
patent: 5008078 (1991-04-01), Yaginuma et al.
patent: 5286624 (1994-02-01), Terashima et al.
Ebbing--General Chemistry, 1984, pp. 494-495.
Benedict et al Journal of Biological Chemistry, vol. 114, 1936, pp. 515-532.
Parekh et al Clinical Chemistry, vol. 23/11, pp. 2066-2071, 1977.
Langley et al Journal of Biological Chemistry, vol. 115, pp. 333-341, 1936.
Parekh et al Clinica Chimica Acta, vol. 73, 1976, pp. 221-231.
Narayanan et al Clinical Chemistry, vol. 26/8, pp. 1119-1126, 1980.

Affiliated with

Also associated with

Rating

Sensitive and highly specific quantitative fluorometric assay fo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sensitive and highly specific quantitative fluorometric assay fo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sensitive and highly specific quantitative fluorometric assay fo will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-222359