Semiselective medium composition for detecting seed-borne Altern

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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4352568, 435431, C12Q 104, C12N 114

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058495169

ABSTRACT:
The present invention provides a novel semiselective medium composition for detecting seed-borne Alternaria brassicicola, which comprises galactose, calcium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, agar, benomyl, and chloramphenicol. The CW medium of the present invention can selectively allow seed-borne Alternaria brassicicola to grow, while effectively suppressing the growth of other seed-borne fungi. Although A. alternata, which is prevalently present on seeds and is similar to A. brassicicola, can still grow on the CW medium, the color of the A. alternata colonies are different from that of A. brassicicola, thus, A. brassicicola can be obviously differentiated.

REFERENCES:
patent: 4375510 (1983-03-01), Jordan
patent: 5573947 (1996-11-01), Madec et al.
Wu et al. in Plant Protection Bulletin 26:67-72 (1984) "A Semiselective Medium for Detecting Seed-borne Alternaria brassicicola".
Wu et al. Plant Protection Bulletin 26:67-72, 1984.
Huang et al. Plant Protection Bulletin 24: 364, 1981.

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