Semi-quantitative assay of metabolic acids

Details Semi-quantitative assay of metabolic acids Semi-quantitative assay of metabolic acids

1981-02-19

1982-09-28

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435805, 435810, C12Q 132

Patent

active

043518990

ABSTRACT:
A test indicator determines concentration, in a biological fluid, of a metabolic acid, such as beta-hydroxybutyrate or lactic acid. The indicator includes a test surface containing the dried residue resulting from impregnation of the surface with components including a tetrazolium salt, nicotinamide adenine dinucleotide (NAD), an electron carrier, and the enzyme that is a dehydrogenase for the specific metabolic acid being assayed. The indicator also includes a physically distinct provision for buffering the reaction. In accordance with the concentration of the metabolic acid being assayed, the indicator provides a predetermined color or intensity which can be observed either with the naked eye or with instrumentation such as a reflectance meter.

REFERENCES:
patent: 3528888 (1970-09-01), Deutsch
patent: 3539453 (1970-11-01), Deutsch
patent: 3867258 (1975-02-01), Forgione
patent: 3957584 (1976-05-01), Kronish et al.

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