Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2005-10-18
2005-10-18
Housel, James (Department: 1648)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023100, C536S023200, C536S024100, C536S024200, C536S024500, C514S04400A, C424S228100, C435S009000, C435S091320, C435S041000, C435S325000, C435S370000, C435S004000
Reexamination Certificate
active
06956117
ABSTRACT:
A unique HCV RNA molecule is provided having an enhanced efficiency of establishing cell culture replication. Novel adaptive mutations have been identified within the HCV non-structural region that improves the efficiency of establishing persistently replicating HCV RNA in cell culture. This self-replicating polynucleotide molecule contains, contrary to all previous reports, a 5′-NTR that can be either an A as an alternative to the G already disclosed and therefore provides an alternative to existing systems comprising a self-replicating HCV RNA molecule. The G->A mutation gives rise to HCV RNA molecules that, in conjunction with mutations in the HCV non-structural region, such as the G(2042)C/R mutations, possess greater efficiency of transduction and/or replication. These RNA molecules when transfected in a cell line are useful for evaluating potential inhibitors of HCV replication.
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Kukolj George
Pause Arnim
Boehringer Ingelheim (Canada) Ltd.
Devlin Mary-Ellen M.
Housel James
Li Bao Qun
Morris Michael P.
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