Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2011-04-05
2011-04-05
Li, Bao (Department: 1648)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S320100, C435S005000, C424S228100
Reexamination Certificate
active
07919312
ABSTRACT:
A unique HCV RNA molecule is provided having an enhanced efficiency of establishing cell culture replication. Novel adaptive mutations have been identified within the HCV non-structural region that improves the efficiency of establishing persistently replicating HCV RNA in cell culture. This self-replicating polynucleotide molecule contains, contrary to all previous reports, a 5′-NTR that can be either an A as an alternative to the G already disclosed and therefore provides an alternative to existing systems comprising a self-replicating HCV RNA molecule. The G→A mutation gives rise to HCV RNA molecules that, in conjunction with mutations in the HCV non-structural region, such as the G(2042)C/R mutations, possess greater efficiency of transduction and/or replication. These RNA molecules when transfected in a cell line are useful for evaluating potential inhibitors of HCV replication.
REFERENCES:
patent: 6630343 (2003-10-01), Bartenschlager
patent: 6689559 (2004-02-01), Wimmer et al.
patent: 6706874 (2004-03-01), Kukolj et al.
patent: 6956117 (2005-10-01), Kukolj et al.
patent: 7344723 (2008-03-01), Kukolj et al.
patent: 2002/0142350 (2002-10-01), Kukolj et al.
patent: 2003/0148348 (2003-08-01), Kukolj et al.
patent: 2303526 (2000-03-01), None
patent: 1043399 (2000-03-01), None
patent: 9804008 (1998-01-01), None
patent: 9839031 (1998-09-01), None
patent: 0066623 (2000-11-01), None
patent: 0189364 (2001-11-01), None
Fournier, Sureau, et al; In vitro infection of adult normal human hepatocytes in primary culture by hepatitis C virus; Journal of General Virology (1998); V. 79; pp. 2367-2374.
Grakoui, Wychowski, et al; Expression and identification of Hepatitis C Virus Polyprotein Cleavage Products; Journal of Virology (Mar. 993); V. 67, No. 3; pp. 1385-1395.
Hijikata, Mizushima, et al; Two Distinct Proteinase Activities Required for the Processing of a Putative Nonstructural Precursor Protein of Hepatitis C. Virus; Journal of Virology (Aug. 1993); V. 67, No. 8; pp. 4665-4675.
Bartenschlager, Ahlborn-Laake, et al; Nonstructural Protein 3 of the Hepatitis C. Virus Encodes a Serine-Type Proteinase Required for Cleavage at the NS3/4 and NS4/5 Junctions; Journal of Virology (Jul. 1993) V. 67, No. 7; pp. 3835-3844.
Reed, Xu, et al; Phosphorylation of the Hepatitis C. Virus NS5A Protein in Vitro and in Vivo: Properties of the NS5A-Associated Kinase; Journal of Virology (Oct. 1997); V. 71, No. 10; pp. 7187-7197.
Khromykh, Westaway; Subgenomic Replicons of the Flavivirus Kunjin: Construction and Applications; Journal of Virology (Feb. 1997); V. 71, No. 2; pp. 1497-1505.
Behrens, Grassmann, et al; Characterization of an Autonomous Subgenomic Pestivirus RNA Replicon; Journal of Virology (Mar. 1998); V. 72, No. 3; pp. 2364-2372.
Mizutani, Kato, et al; Characterization of Hepatitis C. Virus Replication in Cloned Cells Obtained from a Human T-Cell Leukemia Virus Type 1-Infected Cell Line, MT-2; Journal of Virology (Oct. 1996); V. 70, No. 10; pp. 7219-7223.
Moser, Tratschin, et al; A Recombinant Classical Swine Fever Virus Stably Expresses a Marker Gene; Journal of Virology (Jun. 1998); V. 72, No. 6; pp. 5318-5322.
Ikeda, Sugiyama, et al; Human hepatocyte clonal cell lines that support persistent replication of hepatitis C virus; Virus Research; 1998, V. 56; pp. 157-167; Elsevier.
Dash, Halim, et al; Transfection of HepG2 Cells with Infectious Hepatitis C. Virus Genome; American Journal of Pathology; 1997, V. 151; 363-373.
Gou, Ju-Tao, et al; Effect of Alpha Interferon on the Hepatitis C Virus Replicon; Journal of Virology (Sep. 2001); vol. 75, No. 18; pp. 8516-8523.
Hijikata, Makoto, et al; Gene Mapping of the Putative Structural Region of the Hepatitis C. Virus Genome by In Vitro Processing Analysis; Proc. Nat'l. Acad. Sci.; 1991; vol. 88; pp. 5547-5551.
Kim, Don Wook, et al; C-Terminal Domain of the Hepatitis C. Virus NS3 Protein Contains an RNA Helicase Activity; Biochemical and Biophysical Research Communications, Oct. 4, 1995, V. 215(1):160-166.
Kim, J-E, et al; Subcellular Localization of Hepatitis C Viral Proteins in Mammalian Cells; Archives of Virology (1999), 144; pp. 329-343.
Kim, J.L., et al; Crystal Structure of the Hepatitis C. Virus NS3 Protease Domain Complexed with a Synthetic NS4A Cofactor Peptide; Cell (Oct. 18, 1996) vol. 87; pp. 343-355.
Krieger, Nicole, et al; Enhancement of Hepatitis C Virus RNA Replication by Cell Culture-Adaptive Mutations; Journal of Virology (May 2001); V. 75, No. 10; pp. 4614-4624.
Kim, Morgenstern, et al; Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding; Structure (1998), V. 6; pp. 89-100.
Yan, Li, et al; Complex of NS3 protease and NS4A peptide of BK strain hepatitis C virus; A 2.2 A resolution structure in a hexagonal crystal form; Protein Science (1998); V. 7; pp. 837-847.
Yao, Hesson, et al; Structure of the hepatitis C virus RNA helicase domain; Nature Structural Biology; 1997, V. 4, No. 6; pp. 463-467.
Ito, Mukaigawa, et al; Cultivation of hepatitis C virus in primary hepatocyte culture from patients with chronic hepatitis C results in release of high tire infectious virus; Journal of General Virology (1996); V. 77; pp. 1043-1054.
Yoo, Selby, et al; Transfection of a Differentiated Human Hepatoma Cell Line (Huh7) with In Vitro-Transcribed Hepatitis C Virus (HCV) RNA and Establishment of a Long-Term Culture Persistently Infected with HCV; Journal of Virology (Jan. 1995); V. 69, No. 1; pp. 32-38.
Ago, Adachi, et al; Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus; Structure; 1999, V. 7; pp. 1417-1426.
Blight, Kolykhalov, et al; Efficient Initiation of HCV RNA Replication in Call Culture; Science; 2000, V. 290; pp. 1972-1974.
Bressanelli, Tomei, et al; Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus; PNAS; 1999, V. 96, No. 23; pp. 13034-13039.
Cho, Ha, et al; Crystal Structure of RNA Helicase from Genotype 1b Hepatitis C Virus; The Journal of Biological Chemistry (1998); V. 273, No. 24; pp. 15045-15052.
Gale Jr., Korth, et al; Evidence that Hepatitis C Virus Resistance to Interferon is Mediated through Repression of the PKR Protein Kinase by the Nonstractural 5A Protein; Virology; 1997, V. 230; pp. 217-227.
Grakoui, McCourt, et al; A second hepatitis C virus-encoded proteinase, Proc. Natl. Acad. Sci. USA; 1993, V. 90; pp. 10583-10587.
Kwong, Kim, et al; Hepatitis C virus NS3/4A protease; Antiviral Research (1998); V. 40; pp. 1-18; Elsevier.
Landford, Sureau, et al; Demonstration of in Vitro Infection of Chimpanzee Hepatocytes with hepatitis C Virus Using Strand-Specific RT/PCR; Virology; 1994, V. 202; pp. 606-614.
Lesburg, Cable, et al; Crystal structure of the RNA-dependent RNA polymerase from hepatitis C virus reveals a fully encircled active site; Nature Structural Biology (1999); V. 6, No. 10; pp. 937-943.
Lohmann, Korner, et al; Replication of Subgenomic Hepatitis C Virus RNAs in a Hepatoma Cell Line; Science (1999); V. 285; pp. 110-113.
Lohmann, Korner, et al; Mutations in Hepatitis C Virus RNAs Conferring Cell Culture Adaptation; Journal of Virology (2001); V. 75, No. 3; pp. 1437-1449.
Love, Parge, et al; The Crystal Structure of Hepatitis C Virus NS3 Proteinase Reveals a Trypsin-like Fold and a Structural Zinc Binding Site; Cell (1996); V. 87, pp. 331-342.
Shimizu, Purcell, et al; Correlation between the infectivity of hepatitis C virus in vivo and its infectivity in vitro; Proc. Natl. Acad. Sci. USA (1993) V. 90, pp. 6037-6041.
Yanagi, St. Claire, et al; In vivo analysis of the 3′ untranslated region of the hepatitis C virus after in vitro mutagenesis of an infectious cDNA clone; Proc. Nat'l. Acad. Sci. USA (1999) V. 96, pp. 2291-2295.
Bartenschlager, Lohmann; Replication of hepatitis C virus; Journal of General Virology (2000), V. 81, pp. 1631-1648.
International Search Report Issued Mar. 3, 2003 for PCT/CA 01/01843 the counterpart of U.S. Appl. No. 10/029,907.
Bartenschlager, Lohmann; Novel cell culture systems for hepatitis C virus; Antiviral Research (2001); V. 52; pp. 1
Kukolj George
Pause Arnim
Boehringer Ingelheim (Canada) Ltd.
Devlin Mary-Ellen M.
Li Bao
Morris Michael P.
Witkowski Timothy X.
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