Chemistry: analytical and immunological testing – Optical result – Spectrum analysis
Patent
1992-10-07
1994-03-29
Housel, James C.
Chemistry: analytical and immunological testing
Optical result
Spectrum analysis
356326, 422 8208, 422 8209, 422 8211, 436178, G01N 2100
Patent
active
052984281
ABSTRACT:
A method for measuring the concentration of a chemical substance by spectrophotometry comprising the steps of placing a sample of a photoreactive substance between the light source and a spectrophotometer, obtaining an absorption spectrum of the substance using a fixed amount of light from the light source, obtaining a second absorption spectrum after a short interval, comparing the two to determine the concentration of the chemical substance from the difference in the spectra. If the chemical substance is not photoreactive, a photoreactive mixture can be made with a photoreactive dye that has photoreactive properties unique to the mixture. Alternatively, an optically transparent substrate can absorb the substance or the dye/substance mixture.
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John W. Moore, Ralph G. Pearson, Kinetics and Mechanism, 1981, pp. 421-422.
O'Rourke Patrick E.
Van Hare David R.
Collins Laura E.
Dixon Harold M.
Housel James C.
Moser William R.
Tumm Brian R.
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