Self-indicating analysis employing stoichiometric chemical subtr

Details Self-indicating analysis employing stoichiometric chemical subtr Self-indicating analysis employing stoichiometric chemical subtr

1992-08-21

1993-04-06

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 25, 435175, 435 26, 435810, C12Q 132, C12N 1118

Patent

active

052003256

ABSTRACT:
An analytical method and test composition for determining whether an analyte is present in a test sample at a predetermined concentration level by measuring or observing a preselected level of indicator response, e.g., color generation. A subtractive reaction is carried out on the test sample prior to the indicator reaction in order to stoichiometrically and specifically consume a predetermined amount of analyte. The resulting indicator reaction generates the preselected level of indicator response only if the analyte is present at the predetermined concentration level or greater. The subtractive reaction is enzymatically specific for the analyte in the reaction mixture and the amount of analyte consumed can be closely controlled by the amount of a regulating coreactant present at initiation. The method enables the determination of analyte without the need for standards or comparators, e.g., color charts, and therefore is self-indicating. In another embodiment, there is provided a method and test composition for improving an otherwise suboptimal assay response due to oversaturation of the response, e.g., color, generated by an indicator reaction.

REFERENCES:
patent: 3964871 (1976-06-01), Hochstrasser
patent: 4490465 (1984-12-01), Limbach et al.
patent: 4629697 (1986-12-01), Limbach et al.
patent: 5032506 (1991-07-01), Palmer et al.
Bergmeyer, "Methods of Enzymatic Analysis", Second English Edition, Verlag Chemie GmbH, Weinheim/Bergstr, 1974 pp. 1415, 496, 468, 448, 655, 1663 and 574, vol. 1, 2, 3 and 4.

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