Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Calorimeter
Patent
1983-09-09
1985-12-31
Turk, Arnold
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Calorimeter
128638, 128749, 422 58, 422 61, 435294, 435296, 435810, 436 66, G01N 2178, G01N 3372, C12M 130
Patent
active
045620431
ABSTRACT:
A tubular test device for testing occult blood in fecal matter using hydrogen peroxide in an ampoule and 3,3',5,5'-tetramethylbenzidine as the chromogen dissolved in chloroform to impregnate a cellulosic pledget which, when dried, is substantially colorless and is introduced adjacent a sampling swab into the device to lie adjacent the ampoule containing dilute hydrogen peroxide and a wetting agent. A special conditioning pre-coating for the pledget before impregnation with liquid chromogen in chloroform is provided consisting of hydroxyethylcellulose dissolved in an aqueous non-volatile acid buffer such as citric acid and sodium citrate to maintain a pH of 5-7, and then dried. The swab takes a sample of fecal matter. A cap is placed over the swab. The ampoule is broken. If fecal blood is present the hydrogen peroxide passes to the pledget aided by capillary action to create an intense green coloration in the cap very rapidly if the pledget is in the cap above the sampling swab and very slowly if the pledget is below the sampling swab. The color developed for a positive test is exceptionally long-lasting in the cap portion when 6-methoxyquinoline is added to the chromogen in chloroform in making the pledget. In contrast to the guaiac test which fades, the color with the enhancer has not faded after 8 months at room temperature, yet the color developed in the pledget in the cap in about 1 to 2 second.
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Ostrow et al., Digestive Diseases, vol. 18, No. 11, pp. 930-940, 1973.
Freake Ronald
Mennen Frederick C.
Hill Jr. Robert J.
Saffitz A. A.
Turk Arnold
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