Self-contained biological indicator

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

Reexamination Certificate

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C435S287600, C435S287700

Reexamination Certificate

active

06924139

ABSTRACT:
A self-contained biological indicator for evaluating the effectiveness of a sterilizer utilizing an anti-microbial gas or liquid fluid. The indicator is comprised of a tubular casing having open ends and defining an interior chamber. A source of viable microorganisms is disposed within the chamber. A frangible ampoule containing a culture medium capable of promoting growth of the microorganism and a detector means capable of reaction with the metabolites of the microorganism to produce a visual indication is disposed within the chamber. The ampule is operable by pressure applied to an external surface thereof to permit the microorganism and the medium to come into contact with each other. A cap assembly is mounted to each end of the tubular casing. Each cap assembly includes a telescoping element movable from a first position defining an opening communicating with the chamber and a second position crushing the ampoule and forming a gas/fluid impermeable seal around the chamber.

REFERENCES:
patent: 4914034 (1990-04-01), Welsh et al.
patent: 5139957 (1992-08-01), Grack
patent: 5167923 (1992-12-01), Van Iperen
patent: 5516648 (1996-05-01), Malchesky et al.
patent: 5736355 (1998-04-01), Dyke et al.
patent: 5770393 (1998-06-01), Dalmasso et al.
patent: 5801010 (1998-09-01), Falkowski et al.

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