Selective template deletion method

Details Selective template deletion method Selective template deletion method

1996-02-06

1998-11-03

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435912, 536 273, 536 2431, 536 2432, 536 2433, 536 245, 536 221, 536 231, C12P 1934, C12Q 168, C07H 2102, C07H 2104

Patent

active

058307125

ABSTRACT:
A novel method for inactivating undesirable members in a nucleic acid sample which comprises undesirable and desirable members of a nucleic acid family. The method includes the step of adding a blocker to the sample which selectively associates with the undesirable members such that they are unable to participate in any further processing of the sample. The method advantageously provides a means whereby desirable members of the nucleic acid family can be processed, i.e. identified, amplified and/or isolated, without interference from undesirable sequence-related members.

REFERENCES:
Dagle et al, "Targeted degradation of mRNA in Xenopus oocytes and embryos directed by modified oligonucleotides: studies of An2 and cyclin in embryogenesis", Nucleic Acids Res. 18(16):4751-4757, 1990.
Seyama et al, "A novel blocker-PCR method for detection of rare mutant alleles in th presence of an excess amount of normal DNA", Nucleic Acids Res. 20(10):2493-2496, 1992.
Buchman et al, "Selective RNA amplification: A novel method using dUMP containing primers and uracil DNA glycosylase", PCR Method and Applications 3(1): 28-31, 1993.

Affiliated with

Also associated with

Rating

Selective template deletion method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Selective template deletion method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Selective template deletion method will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-688289