Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Patent
1990-10-23
1992-01-28
Moskowitz, Margaret
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
514 12, 530324, 530387, 436538, 604 6, G01N 33544, G01N 33537, A61M 3700, A61K 3702
Patent
active
050843982
ABSTRACT:
Disclosed is a method and a family of materials useful for removing immune complexes from blood preferentially to soluble antibodies. The material comprises analogs of proteins which bind to the Fc region of immunoglobulin. The analogs are produced by truncating or otherwise altering the amino acid sequence of the binding protein to reduce their affinity for Fc. An array of such analogs disposed about the surface of an insoluble matrix has the ability to form multiple points of attachment to the multiple Fc's in a complex so as to bind complex strongly, whereas only weak associations are developed between the Fc region of soluble IgG and inidivdual analogs. The preferred analogs are truncated proteins homologous to a portion of the domains of Protein A or Protein G which bind with Fc. Complex may be removed from whole blood or serum using the material and conventional plasmapheresis techniques.
REFERENCES:
patent: 4614513 (1986-09-01), Bensinger
patent: 4687734 (1987-08-01), Chester
Uhlen et al. (Gene (1983) 23:369-378.
Selective Affinity of Protein A Containing Staphylococci for Monomeric and Polymeric IgG, Maria-Ana Dobre et al., Journal of Immunological Methods, vol. 66, pp. 171-78 (1984).
Baird Lynn
Cohen Charles
Huston James S.
Oppermann Hermann
Creative BioMolecules
Guest Shelly J.
Moskowitz Margaret
Pitcher Edmund R.
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