Selective precipitation of viruses

Details Selective precipitation of viruses Selective precipitation of viruses

2005-04-26

2005-04-26

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

Recovery or purification

C435S235100, C210S729000, C210S767000

Reexamination Certificate

active

06884613

ABSTRACT:
The invention provides new methods for purifying and concentrating viruses. The inventors have discovered that high molecular weight proteoglycans present in retroviral stocks are co-concentrated with the retroviruses, and can inhibit retroviral transduction. The new purification and concentration methods feature treatment of virus stock with an anionic polyelectrolyte and a cationic polyelectrolyte, followed by centrifugation. The new methods minimize the amount of proteoglycan co-precipitated with the infectious virus.

REFERENCES:
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patent: 0 870 839 (1998-10-01), None
Arcasoy et al. (1997) Gene Therapy 4:32-38.
Le Doux et al. (1998) Biotechnology and Bioengineering 58(1):23-34.
Nguyen et al.. (1987) Veterinary Microbiology 14:343-354.
Superti et al. (1993) Comp. Immun. Microbiol. Infect. Dis. 16(1):55-62.
Wallis et al. (1968) Journal of Virology 2(4):267-274.

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