Selective growth medium for isolation of Mobiluncus from vaginal

Details Selective growth medium for isolation of Mobiluncus from vaginal Selective growth medium for isolation of Mobiluncus from vaginal

1987-01-14

1989-06-06

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 38, 435871, C12N 120, C12Q 110, C12R 136

Patent

active

048371548

ABSTRACT:
A selective growth medium for the isolation of Mobiluncus includes a medium base including an aqueous preparation of a gelling agent and a nutrient broth effective for culturing Mobiluncus. The selective growth medium of the invention further includes a first antimicrobial agent in effective antimicrobial amounts, the antimicrobial agent being selected from the group consisting of colistin, nalidixic acid, and combinations of colistin and nalidixic acid, the colistin having a concentration less than 32 micrograms/ml and the nalidixic acid having a concentration less than 100 micrograms/ml. Tinidazole is included as a second antimicrobial agent in effective, antimicrobial amounts not greater than 1.0 micrograms/ml. Nile Blue A is also included in Gardnerella Vaginalis inhibiting concentrations.

REFERENCES:
E. Hjelm, et al. (1984), "Primary Isolation of Curvest Rods from Women With Vaginal Discharge," Scand. J. Nephrol. Urol., Suppl. 86, 113-116.
E. Holst, et al. (1984), "Anaerobic Curved Rods in Genital Samples of Women," Scand. J. Nephrol. Urol., Suppl., 86, 117-124.
J. L. Thomason, et al. (1984), "A Selective and Differential Agar for an Anaerobic Comma-shaped Bacteria Recovered from Patients Having Motile Rods and Non-specified Vaginosis," Scand. J. Nephrol. Urol., Suppl., 86, 125-128.
A. Fox, et al. (1984),: "Two Curved Rods in Non-Specific Vaginitis," Scand. J. Nephrol. Urol., Suppl., 86, 93-96.
C. Pahlson, et al. (1984), "Numerical Taxonomy of Motile Anaerobic Curved Rods Isolated from Vaginal Discharge," Scand. J. Nephrol. Urol., Suppl., 86, 251-256.
M. Sprott, et al. (1984), "Motile Curved Bacilli," Scand. J. Nephrol. Urol., Suppl, 86, 107-111.
A. J. Taylor, et al. (1984), "Morphological and Chemical Characteristics of an Aerobic Curved Rod-Shaped Bacteria From the Female Genital Tract," Scand. J. Nephrol. Urol., Suppl., 86, 97-106.
M. Sprott, et al. (1983), "Characteristics of Motile Curved Rods in Vaginal Secretions," J. Med. Mocrobiol., 16, 175-182.
C. Pahlson, et al. (1983), "Characterization of Motile Anaerobic Curved Rods Isolated from Women with Lower Genital Tract Infection in Three Different Countries," Eur. J. Sex. Transm., 73-75.
E. Holst, et al. (1982), "Characteristics of Anaerobic Comma-Shaped Bacteria Recovered from the Female Genital Tract," Eur. J. Clin. Microbiol., 1, 310-116.
A. Skarin, et al. (1983), "Antimicrobial Susceptibility of Comma-shaped Bacteria Isolated from the Vagina," Scand. J. Infect. Dis., Suppl., 40, 81-84.
E. Holst, et al. (1983), "Anaerobic comma-shaped Bacteria Recovered from the Human Genital Tract, A Review," Scand. J. Infect. Dis., Suppl. 40, 23-30.

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