Selective determination of nucleotides in viable somatic and mic

Details Selective determination of nucleotides in viable somatic and mic Selective determination of nucleotides in viable somatic and mic

1979-11-30

1981-12-01

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 18, 435 29, 435 34, 435820, C12Q 106, C12Q 166, C12Q 134

Patent

active

043037526

ABSTRACT:
A method is disclosed for measuring the concentration of nucleotides and the number of viable cells in a biological sample containing somatic and/or microbial cells by treatment of a sample of cells with surface active agents to selectively release the nucleotides without rupture of the cell membrane or walls, and measuring by means of a bioluminescent assay technique the concentration of nucleotides selectively released and thereby determining the number of viable cells in the sample.

REFERENCES:
patent: 3645852 (1972-02-01), Axen et al.
patent: 3745090 (1973-07-01), Chappelle et al.
patent: 3788948 (1974-01-01), Kagedal et al.
patent: 3930956 (1976-01-01), Juni
patent: 3971703 (1976-07-01), Picciolo et al.
patent: 4014745 (1977-03-01), Fletcher et al.
patent: 4144134 (1979-03-01), Chris Jr.
Reese et al., Surfactants as Stimulants of Enzyme Production By Microorganisms, Applied Microbiology, vol. 17, No. 2, 1969, (pp. 242-245).

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