Selective detection of viable and infectious cryptosporidium ooc

Details Selective detection of viable and infectious cryptosporidium ooc Selective detection of viable and infectious cryptosporidium ooc

1995-03-14

1996-09-17

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 536 243, 536 2432, C12P 1934, C12Q 168, C07H 2104

Patent

active

055567746

ABSTRACT:
With the help of PCR it is possible to detect parasites of the genus Cryptosporidium by amplifying specific gene sequences. The disadvantage of this method is that it does not allow a statement about whether the detected gene sequences have been derived from viable and infectious parasites or from dead organisms or even from free DNA which has been released from organisms which have already disintegrated. However, by the combination of PCR with other known methods, i.e. an in vitro excystation assay and the removing or the destruction of nucleic acids which might be present in a sample material outside of Cryptosporidium oocysts before excystation this problem can be solved. Due to this combination the result of a PCR for Cryptosporidium allows a judgement on the infectivity of the examined material which would not be possible without this combination. Thus the fundamental advantages of PCR (high sensitivity and specificity, objective results, possibility of processing several samples in parallel) become available for applications in the field of environmental and food hygiene where it is essential to be able to answer the question whether a sample material is infectious or not.

REFERENCES:
C. E. Chrisp and M. LeGendre, Folia Parasitologica 41:97-100.
Laxer et al., Am. J. Trop. Med. Hyg 45: 688-694.
Johnson et al. Wat. Sci. Tech. 27: 77-84.

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