Selective detection of mycobacteria by nucleicacid probes derive

Details Selective detection of mycobacteria by nucleicacid probes derive Selective detection of mycobacteria by nucleicacid probes derive

1993-05-26

1994-10-04

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 9152, 536 2432, 536 2433, 935 78, C12Q 168, C12P 1934, C07H 2104

Patent

active

053525809

ABSTRACT:
Oligonucleotide probes derived from Mycobacteria Kansasii capable of selectively hybridizing to Mycobacteria nucleic acid are disclosed. The oligonucleotide probe is selected from the group consisting of: (a) an oligonucleotide probe consisting essentially of the DNA sequence given herein as SEQ ID NO:1 (MK14); (b) oligonucleotide probes comprising fragments of MK14 which retain the capability of MK14 of selectively hybridizing to Mycobacteria nucleic acid; (c) oligonucleotide probes which hybridize to MK14 and are capable of selectively hybridizing to Mycobacteria nucleic acid; and (d) oligonucleotide probes which are complementary to any of the foregoing and are capable of selectively hybridizing to Mycobacteria nucleic acid. The probes are useful in nucleic acid amplification and hybridization assays for genus-specific detection of the mycobacteria, specific detection of M. kansasii and specific detection of the slow-growing mycobacteria. Methods of using the probes to detect and amplify Mycobacteria nucleic acid and kits containing the same are also disclosed.

REFERENCES:
patent: 5168039 (1992-12-01), Crawford et al.
Z. H. Huang, et al. "Identification of Mycobacterium kansasii by DNA Hybridization" J. Clin. Microbiol. 29:2125-2129 (1991).
J. W. Fries, et al. "Genus- and species-specific DNA probes to identify mycobacteria using the polymerase chain reaction" Molec. Cell. Probes 4:87-105 (1990).
A. J. Hance, et al. "Detection and identification of mycobacteria by amplification of mycobacterial DNA" Molec. Microbiol. 3:843-849 (1989).
Spears et al. Abstracts of the General Meeting--1992: Abstract No. 04, ASM, 1992.

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