Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-08-30
1993-11-23
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 724, 435 30, 4351722, 43524027, 435948, 436548, C12N 1502, G01N 33577
Patent
active
052643417
ABSTRACT:
The invention is a method for selectively isolating cells which secrete higher levels of monoclonal antibody than did the parental culture from which they were derived. The method is based on the membrane characteristics of the cell and requires the use of a cell sorter. At present, the invention's best mode utilizes fluorescent probes in conjunction with a fluorescence activated cell sorter. The method is based on the correlation of a high level of cell surface immunoglobulin with high monoclonal antibody secretion rates. The invention provides a predictable and rapid process for selecting high monoclonal antibody producing cells.
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Francois Martel et al., Characterization of Higher Avidity Monoclonal Antibodies Produced by Murine B-Cell Hybridoma Variants Selected for Increased Antigen Binding of Membrane Ig.sup.1, The Journal of Immunology, vol. 141, No. 5, pp. 1624-1629, (Sep. 1988).
Eliane Meilhoc et al., Application of Flow Cytometric Measurement of Surface IgG in Kinetic Analysis of Monoclonal Antibody Synthesis and Secretion by Murine Hybridoma Cells, Journal of Immunological Methods, vol. 121, pp. 167-174, (1989).
Maciak Ronald S.
Marder Philip
Eli Lilly and Company
Maciak Ronald S.
Saunders David
Whitaker Leroy
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