Selective attachment of nucleic acid molecules to patterned self

Details Selective attachment of nucleic acid molecules to patterned self Selective attachment of nucleic acid molecules to patterned self

1994-12-01

1997-11-18

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 911, 435 912, 435176, 204403, 536 243, 536 2433, 536 2432, 536 253, 356445, C12Q 168, C12Q 170, C12P 1934, C07H 2104

Patent

active

056886420

ABSTRACT:
Patterns of pre-formed hybridizable nucleic acid oligomers are formed upon a substrate. The substrate is coated with molecules, such as aminosilanes, whose reactivity with nucleic acid molecules can be transformed by irradiation. The coated substrate exposed to patterned irradiation then contacted with pre-formed nucleic acid oligomers. The binding of the preformed nucleic acid oligomers to the coating molecules may be covalent or non-covalent (for example, ionic bonding or hydrogen bonding). If desired, a heterobifunctional crosslinker may be employed, before or after irradiation, with the coating to promote covalent binding of the nucleic acid oligomers to the coating molecules. Also, the irradiation step may be performed with the assistance of a positive-tone or negative-tone photoresist.

REFERENCES:
patent: 4562157 (1985-12-01), Lowe et al.
patent: 5077085 (1991-12-01), Schnur et al.
patent: 5077210 (1991-12-01), Eigler
patent: 5079600 (1992-01-01), Schnur et al.
patent: 5200051 (1993-04-01), Cozzette et al.
patent: 5252743 (1993-10-01), Barrett et al.
patent: 5391463 (1995-02-01), Ligler et al.
patent: 5482830 (1996-01-01), Bogart et al.
Chrisey et al., Mat. Res. Soc. Symp. Proc. vol. 330, pp. 179-184 (1994).
Fodor et al., Science, vol. 251, pp. 767-773 (1991).
Bhatia et al., Anal. Bioch., 208, pp. 001-012 (1993).
Kawai et al., Anal. Bioch., 209, pp. 63-69 (1993).
Seiki et al., Proc. Natl. Acad. Sci. USA, vol. 86, pp. 6230-6234 (1989).
Calvert, Jeff M., J. Voac. Sci. Technol. B 11(6), Nov./Dec. 1993, pp. 215163.
Calvert, Jeff M., "Lithographically Patterned Self-Assembled Films", Thin Films, vol. 20: Organic Thin Films and Surfaces, A. Ulman, Ed., Academic Press, Boston (1995), 109-141. (Invited Paper).

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