Selective amplification of target polynucleotide sequences

Details Selective amplification of target polynucleotide sequences Selective amplification of target polynucleotide sequences

1995-06-06

2000-07-18

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 911, 536 243, C12P 1934, C12Q 168, C07H 2104

Patent

active

060905913

ABSTRACT:
A method is provided for multiplying the number of copies of a target polynucleotide sequence comprising a series of primer hybridization, extending, and denaturing steps to provide an intermediate double-stranded DNA molecule containing a promoter sequence (through the use of a promoter-sequence-containing primer) incorporated upstream from the target sequence. The double-stranded DNA intermediate is then used to grow multiple RNA copies of the target sequence. The resulting RNA copies can be used as target sequences to produce further copies. Multiple cycles of this sort can thereby exponentially increase the number of target sequence copies.

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