Selective amplification of target polynucleotide sequences

Details Selective amplification of target polynucleotide sequences Selective amplification of target polynucleotide sequences

1993-09-13

1995-08-01

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 911, 435 9121, 435 913, 435 915, 435 9151, 436501, 536 221, 536 231, 536 253, 935 88, C12P 1934, C07H 2102, C07H 2104

Patent

active

054379903

ABSTRACT:
A method is provided for multiplying the number of copies of a target polynucleotide sequence comprising a series of primer hybridization, extending, and denaturing steps to provide an intermediate double-stranded DNA molecule containing a promoter sequence (through the use of a promoter-sequence-containing primer) incorporated upstream from the target sequence. The double-stranded DNA intermediate is then used to grow multiple RNA copies of the target sequence. The resulting RNA copies can be used as target sequences to produce further copies. Multiple cycles of this sort can thereby exponentially increase the number of target sequence copies.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5130238 (1992-07-01), Malek et al.
Mullis, Kary B.; slide of May 1986 talk at the University of California Berkley.
Guatelli et al. Proc Natl Acad Sci 87:1874-78 (1990).
Genetic Engineering News Jun. 1992 pp. 1, 8 & 9.
Melton et al. Nuc Acids Res 12(18) pp. 7035-7056 (1984).
V. D. Axelrod et al., Transcription from Bacteriophage T7 and SP6 RNA Polymerase Promoters in the Presence of 3'-Deoxyribonucleoside 5'-Triphosphate Chain Terminators, Biochemistry (1985) 24:5716-5723.

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