Selective adsorbent for use in affinity chromatography

Details Selective adsorbent for use in affinity chromatography Selective adsorbent for use in affinity chromatography

1976-07-06

1977-12-06

Shine, W. J.

Compositions

Preservative agents

Anti-oxidants or chemical change inhibitants

252428, B01J 3102

Patent

active

040615910

ABSTRACT:
A new selective adsorbent for use in affinity chromatography. This adsorbent comprises, as one part, a water soluble covalently bonded complex of an affinity ligand and a biospecific handle. The other part thereof is an insoluble matrix or solid support which has covalently immobilized on its surface an adsorbent which has specific affinity for the biospecific handle of the complex. Attachment between the complex, containing the ligand, and the support is achieved by reversible coupling between the adsorbent and the biospecific handle and, as a result thereof, the ligand is not covalently bonded to the support. In turn, substantially complete recovery of the enzyme from the column can be effected.

REFERENCES:
philipson et al., Proc. Nat. Acad. Sci. U.S.A., 68, pp. 2806-2809 (1971).
Sheldon et al., Proc. Nat. Acad. Sci. U.S.A., 69, pp. 417-421 (1972).
"Poly(U)-Sepharose 4B", Pharmacia Fine Chemicals AB, Jan. 1974.

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