Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2001-09-12
2004-09-28
Winkler, Ulrike (Department: 1648)
Chemistry: molecular biology and microbiology
Vector, per se
C536S023100, C536S023400, C536S023720, C536S024100, C536S024200
Reexamination Certificate
active
06797512
ABSTRACT:
BACKGROUND OF THE INVENTION
Generation of efficient packaging cell lines for lentiviral vectors is hampered by the cytotoxicity of some of the products of the gag and pol genes. Thus, it is desirable to have inducible expression of gag and pol so that optimal clones that will express gag and pol at high levels when needed can be selected in the absence of gag/pol expression.
SUMMARY OF THE INVENTION
A method for selecting cells which express gag and pol and thus are useful as packaging cells is obtained by linking a selectable marker to the gag/pol expression cassette of a packaging vector in such a way that the marker is expressed by the same promoter which controls expression of the gag/pol genes although expression of the gag/pol genes is suppressed. Efficient expression of the marker predicts efficient expression of the gag/pol genes on induction.
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Ory, Daniel S. et al., “A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes,” Proc. Natl. Acad. Sci., USA, vol. 93, Oct. 1996, pp. 11400-11406.
Schneider, Ralf et al., “Inactivation of the Human Immunodeficiency Virus Type 1 Inhibitory Elements Allows Rev-Independent Expression of Gag and Gag/Protease and Particle Formation,” Journal of Virology, vol. 71, No. 7, Jul. 1997, pp. 4892-4903.
Yu et al., “Inducible Human Immunodeficiency Virus Type 1 Packaging Cell Lines,” Jnl of Virology, 1996, 70(7): 4530-4537.
McGuinness Ryan
Naldini Luigi
Cell Genesys Inc.
Gates & Cooper LLP
Judge Linda R.
Winkler Ulrike
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